Rabbit FBXO5 Polyclonal Antibody | anti-FBXO5 antibody

Anti-FBXO5 Antibody

Cat. Num. AAA417315

• Gene Names: FBXO5; EMI1; FBX5; Fbxo31
• Reactivity: Human. Predicted: Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity purified
• Synonyms: FBXO5; Polyclonal Antibody; Anti-FBXO5 Antibody; anti-FBXO5 antibody

• Host: Rabbit
• Reactivity: Human. Predicted: Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 2034

• Applicable Applications for anti-FBXO5 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA titer: 1:20,000-1:80,000; Western blot analysis: 1:200-1:1,000; Optimal dilutions/concentrations should be determined by the end user
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Immunogen: Rabbit polyclonal FBXO5 (1) antibody was raised against a recombinate human FBXO5 protein 272-438aa (BC018905)
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-FBXO5 antibody

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. This gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Dec 2008]

References

(1) Zhao,Y., Tang,Q., Ni,R., et al. Early mitotic inhibitor-1, an anaphase-promoting complex/cyclosome inhibitor, can control tumor cell proliferation in hepatocellularcarcinoma: correlation with Skp2 stability and degradation of p27(Kip1). Hum. Pathol. 44 (3), 365-373 (2013)

NCBI and Uniprot Product Information

• NCBI GI #: 17511910
• NCBI GeneID: 26271
• Molecular Weight: 45,353 Da
• NCBI Official Full Name: Homo sapiens F-box protein 5, mRNA
• NCBI Official Synonym Full Names: F-box protein 5
• NCBI Official Symbol: FBXO5
• NCBI Official Synonym Symbols: EMI1; FBX5; Fbxo31
• NCBI Protein Information: F-box only protein 5; F-box protein Fbx5; early mitotic inhibitor 1
• UniProt Protein Name: F-box only protein 5
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO5
• UniProt Entry Name: FBX5_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Dec 2008]

Uniprot Description

FBX5: a member of the SCFs class of F-box proteins. F-box proteins constitute one of the subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. FBX5 is an early mitotic inhibitor-1 (Emi1) that interacts with Cdc20 and inhibits the anaphase promoting complex (APC/C). APC/C is a ubiquitin ligase that targets mitotic regulatory proteins for degradation. FBX5 accumulates in the S phase and is rapidly degraded in prometaphase.

Chromosomal Location of Human Ortholog: 6q25.2

Cellular Component: nucleoplasm; cytoplasm; spindle; cytosol; nucleus

Molecular Function: protein binding; metal ion binding; protein kinase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; vesicle organization and biogenesis; regulation of mitotic cell cycle; oocyte maturation; G1/S-specific transcription in mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; negative regulation of meiosis; mitotic cell cycle; spindle assembly involved in female meiosis I; mitotic metaphase/anaphase transition; microtubule polymerization; G1/S transition of mitotic cell cycle