SDS-Page
PFDN6 recombinant protein
PFDN6, 1-129aa, Human, His tag, E Coli
Gene Names
PFDN6; HKE2; KE-2; PFD6; H2-KE2
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
PFDN6; 1-129aa; Human; His tag; E Coli; Prefoldin subunit 6; H2-KE2; HKE2; KE-2; MGC70744; PFD6; PFDN6 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol
Concentration
0.5 mg/ml (determined by BCA assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MAELIQKKLQ GEVEKYQQLQ KDLSKSMSGR QKLEAQLTEN NIVKEELALL DGSNVVFKLL GPVLVKQELG EARATVGKRL DYITAEIKRY ESQLRDLERQ SEQQRETLAQ LQQEFQRAQA AKAGAPGKA
Sequence Length
129
Applicable Applications for PFDN6 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for PFDN6 recombinant protein
PFDN6, also known as HKE2 or PFD6, is a subunit of the heteromeric prefoldin complex that chaperones nascent actin and alpha- and beta-tubulin chains pending their transfer to the cytosolic chaperonin containing TCP1 (CCT) complex. It binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it and binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Recombinant human PFDN6 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for PFDN6 recombinant protein
References
Sowa ME., et al. (2009) Cell. 138 (2):389-403.; Simons CT., et al. (2004) J Biol Chem. 279 (6):4196-203.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16.7 kDa (149aa) confirmed by MALDI-TOF
NCBI Official Full Name
prefoldin subunit 6
NCBI Official Synonym Full Names
prefoldin subunit 6
NCBI Official Symbol
PFDN6
NCBI Official Synonym Symbols
HKE2; KE-2; PFD6; H2-KE2
NCBI Protein Information
prefoldin subunit 6
UniProt Protein Name
Prefoldin subunit 6
Protein Family
UniProt Gene Name
PFDN6
UniProt Synonym Gene Names
HKE2; PFD6
NCBI Description
PFDN6 is a subunit of the heteromeric prefoldin complex that chaperones nascent actin (see MIM 102560) and alpha- and beta-tubulin (see MIM 602529 and MIM 191130, respectively) chains pending their transfer to the cytosolic chaperonin containing TCP1 (MIM 186980) (CCT) complex (Hansen et al., 1999 [PubMed 10209023]).[supplied by OMIM, Jul 2010]
Uniprot Description
Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
Research Articles on PFDN6
Similar Products
Product Notes
The PFDN6 pfdn6 (Catalog #AAA203903) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's PFDN6 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the PFDN6 pfdn6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MAELIQKKLQ GEVEKYQQLQ KDLSKSMSGR QKLEAQLTEN NIVKEELALL DGSNVVFKLL GPVLVKQELG EARATVGKRL DYITAEIKRY ESQLRDLERQ SEQQRETLAQ LQQEFQRAQA AKAGAPGKA. It is sometimes possible for the material contained within the vial of "PFDN6, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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