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Western Blot (WB) (Western Blot: Sample: Rat Pancreas lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ELA2A Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Mouse Elastase 2A Polyclonal Antibody | anti-ELA2A antibody

Polyclonal Antibody to Elastase 2A (ELA2A)

Gene Names
Cela2a; Ela2; Ela-2; Ela2a
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Elastase 2A; Polyclonal Antibody; Polyclonal Antibody to Elastase 2A (ELA2A); anti-ELA2A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ELA2A. It has beenselected for its ability to recognize ELA2A in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/ml (varies by lot)
Sequence Length
269
Applicable Applications for anti-ELA2A antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
ELA2A (Cys17~Asn271)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse, Rat
Conjugate
No Conjugate
Immunogen
Recombinant ELA2A (Cys17~Asn271) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2074137
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Rat Pancreas lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ELA2A Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Rat Pancreas lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ELA2A Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Mouse Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P. Samples: Mouse Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,914 Da
NCBI Official Full Name
chymotrypsin-like elastase family member 2A preproprotein
NCBI Official Synonym Full Names
chymotrypsin-like elastase family, member 2A
NCBI Official Symbol
Cela2a
NCBI Official Synonym Symbols
Ela2; Ela-2; Ela2a
NCBI Protein Information
chymotrypsin-like elastase family member 2A
UniProt Protein Name
Chymotrypsin-like elastase family member 2A
UniProt Gene Name
Cela2a
UniProt Synonym Gene Names
Ela-2; Ela2; Ela2a

NCBI Description

This gene encodes a serine protease enzyme that hydrolyzes elastin. This gene is highly expressed in the pancreatic acinar cells where the encoded preproprotein undergoes processing including signal peptide cleavage to generate an inactive zymogen. The removal of N-terminal activation peptide from the zymogen by trypsin generates active elastase enzyme. This gene is also expressed in the mouse epidermis where it participates in pro-filaggrin processing. [provided by RefSeq, Jul 2016]

Uniprot Description

CELA2A: Acts upon elastin. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: EC 3.4.21.71; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4 E1|4 74.75 cM

Cellular Component: extracellular region; extracellular space

Molecular Function: hydrolase activity; peptidase activity; serine hydrolase activity; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: proteolysis

Research Articles on ELA2A

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Product Notes

The ELA2A cela2a (Catalog #AAA2026612) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Elastase 2A (ELA2A) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Elastase 2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the ELA2A cela2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Elastase 2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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