Western Blot (WB)
(Western Blot: Sample: Mouse Eye lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse CRYbA1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Rabbit anti-Mouse Crystallin Beta A1 Polyclonal Antibody | anti-CRYbA1 antibody
Polyclonal Antibody to Crystallin Beta A1 (CRYbA1)
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Crystallin Beta A1; Polyclonal Antibody; Polyclonal Antibody to Crystallin Beta A1 (CRYbA1); anti-CRYbA1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CRYbA1. It has beenselected for its ability to recognize CRYbA1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
215
Applicable Applications for anti-CRYbA1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
CRYbA1 (Met1~Gln215)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse, Rat, Pig
Conjugate
No Conjugate
Immunogen
Recombinant CRYbA1 (Met1~Gln215) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2076309
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Eye lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse CRYbA1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Mouse Eye lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse CRYbA1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
23,401 Da
NCBI Official Full Name
Beta-crystallin A1
UniProt Protein Name
Beta-crystallin A1
Protein Family
UniProt Gene Name
Cryba1
Uniprot Description
CRYBA1: a major structural protein of the eye lens. A member of the beta/gamma-crystallin family. Defects are the cause of congenital zonular cataract with sutural opacities. Two splice-variant isoforms have been described.
Chromosomal Location of Human Ortholog: 11 B5|11 46.74 cM
Cellular Component: cytoplasm; nucleus
Molecular Function: protein homodimerization activity; structural constituent of eye lens
Biological Process: camera-type eye development; negative regulation of cytokine production; negative regulation of ERK1 and ERK2 cascade; negative regulation of phosphatidylinositol 3-kinase signaling; negative regulation of protein kinase B signaling cascade; negative regulation of TOR signaling; positive regulation of anoikis; visual perception
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Product Notes
The CRYbA1 cryba1 (Catalog #AAA2026366) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Crystallin Beta A1 (CRYbA1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Crystallin Beta A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the CRYbA1 cryba1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Crystallin Beta A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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