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Western Blot (WB) (Western Blot: Sample: Rat Eye lysate; Primary Ab: 0.5ug/ml Rabbit Anti-Mouse CRYbB1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Mouse Crystallin Beta B1 Polyclonal Antibody | anti-CRYbB1 antibody

Polyclonal Antibody to Crystallin Beta B1 (CRYbB1)

Gene Names
Crybb1; BB1CRY; 3110006K12Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Crystallin Beta B1; Polyclonal Antibody; Polyclonal Antibody to Crystallin Beta B1 (CRYbB1); anti-CRYbB1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CRYbB1. It has beenselected for its ability to recognize CRYbB1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
252
Applicable Applications for anti-CRYbB1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
CRYbB1 (Lys6~Val186)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse, Rat
Conjugate
No Conjugate
Immunogen
Recombinant CRYbB1 (Lys6~Val186) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2076315
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Rat Eye lysate; Primary Ab: 0.5ug/ml Rabbit Anti-Mouse CRYbB1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Rat Eye lysate; Primary Ab: 0.5ug/ml Rabbit Anti-Mouse CRYbB1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded liver tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded liver tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,003 Da
NCBI Official Full Name
beta-crystallin B1
NCBI Official Synonym Full Names
crystallin, beta B1
NCBI Official Symbol
Crybb1
NCBI Official Synonym Symbols
BB1CRY; 3110006K12Rik
NCBI Protein Information
beta-crystallin B1
UniProt Protein Name
Beta-crystallin B1
Protein Family
UniProt Gene Name
Crybb1

NCBI Description

This gene encodes a member of the crystallin family of proteins that contribute to the transparency and refractive properties of the ocular lens. The encoded protein associates with other beta crystallin proteins to form dimers, tetramers and other higher-order complexes. This gene is located adjacent to a related crystallin gene on chromosome 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

CRYBB1: a major structural protein of the eye lens. A member of the beta/gamma-crystallin family. Specific cleavages in the N-terminal arm occur during lens maturation and give rise to truncated forms, leading to impaired oligomerization and protein insolubilization. The protease responsible for this partial degradation could be calpain II.

Chromosomal Location of Human Ortholog: 5 F|5 54.63 cM

Molecular Function: protein binding; structural constituent of eye lens

Biological Process: visual perception

Research Articles on CRYbB1

Similar Products

Product Notes

The CRYbB1 crybb1 (Catalog #AAA2027815) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Crystallin Beta B1 (CRYbB1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Crystallin Beta B1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the CRYbB1 crybb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Crystallin Beta B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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