Mouse Elastase 2A (ELA2A) RTU ELISA Kit | ELA2A rtu elisa kit

Mouse Elastase 2A (ELA2A) ELISA Kit

Cat. Num. AAA459027

• Gene Names: Cela2a; Ela2; Ela-2; Ela2a
• Reactivity: Mouse
• Synonyms: Elastase 2A (ELA2A); Mouse Elastase 2A (ELA2A) ELISA Kit; ELA1; PE-1; CELA2A; Chymotrypsin-Like Elastase Family Member 2A; ELA2A rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 271

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 31.2-2000pg/mL
• Sensitivity: 12.8pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ELA2A rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ELA2A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ELA2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ELA2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ELA2A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6681297
• NCBI GeneID: 13706
• NCBI Accession #: NP_031945.1
• NCBI GenBank Nucleotide #: NM_007919.3
• UniProt Accession #: P05208
• Molecular Weight: 28,914 Da
• NCBI Official Full Name: chymotrypsin-like elastase family member 2A preproprotein
• NCBI Official Synonym Full Names: chymotrypsin-like elastase family, member 2A
• NCBI Official Symbol: Cela2a
• NCBI Official Synonym Symbols: Ela2; Ela-2; Ela2a
• NCBI Protein Information: chymotrypsin-like elastase family member 2A
• UniProt Protein Name: Chymotrypsin-like elastase family member 2A
• UniProt Gene Name: Cela2a
• UniProt Synonym Gene Names: Ela-2; Ela2; Ela2a

NCBI Description

This gene encodes a serine protease enzyme that hydrolyzes elastin. This gene is highly expressed in the pancreatic acinar cells where the encoded preproprotein undergoes processing including signal peptide cleavage to generate an inactive zymogen. The removal of N-terminal activation peptide from the zymogen by trypsin generates active elastase enzyme. This gene is also expressed in the mouse epidermis where it participates in pro-filaggrin processing. [provided by RefSeq, Jul 2016]

Uniprot Description

Acts upon elastin.