Western Blot (WB)
(Western Blot: Sample: Recombinant MIP1a, Rat.)
Rabbit anti-Rat Macrophage Inflammatory Protein 1 Alpha Polyclonal Antibody | anti-MIP1a antibody
Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a)
Gene Names
Ccl3; Scya3; MIP-1a
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Macrophage Inflammatory Protein 1 Alpha; Polyclonal Antibody; Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a); anti-MIP1a antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MIP1a. It has beenselected for its ability to recognize MIP1a in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
0.49mg/ml (varies by lot)
Sequence Length
70
Applicable Applications for anti-MIP1a antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Fragment
MIP1a (Ala24~Ala92)
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Rat
Conjugate
No Conjugate
Immunogen
Recombinant MIP1a (Ala24~Ala92) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2037563
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant MIP1a, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant MIP1a, Rat.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,335 Da
NCBI Official Full Name
C-C motif chemokine 3
NCBI Official Synonym Full Names
C-C motif chemokine ligand 3
NCBI Official Symbol
Ccl3
NCBI Official Synonym Symbols
Scya3; MIP-1a
NCBI Protein Information
C-C motif chemokine 3
UniProt Protein Name
C-C motif chemokine 3
Protein Family
UniProt Gene Name
Ccl3
UniProt Synonym Gene Names
Mip1a; Scya3; MIP-1-alpha
NCBI Description
mediates monocyte and neutrophil chemotaxis; may play a role in the pathogenesis of acute lung injury [RGD, Feb 2006]
Uniprot Description
Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.
Research Articles on MIP1a
Similar Products
Product Notes
The MIP1a ccl3 (Catalog #AAA2026439) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Macrophage Inflammatory Protein 1 Alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the MIP1a ccl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Macrophage Inflammatory Protein 1 Alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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