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Western Blot (WB) ((0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat Stomatin Polyclonal Antibody | anti-STOM antibody

Goat anti-Stomatin Antibody

Gene Names
STOM; BND7; EPB7; EPB72
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Stomatin; Polyclonal Antibody; Goat anti-Stomatin Antibody; STOM; stomatin; BND7; EPB7; EPB72; erythrocyte band 7 integral membrane protein; erythrocyte membrane protein band 7.2 (stomatin); protein 7.2b; STOM antibody; stomatin antibody; BND7 antibody; EPB7 antibody; EPB72 antibody; erythrocyte band 7 integral membrane protein antibody; erythrocyte membrane protein band 7.2 (stomatin) antibody; protein 7.2b antibody; anti-STOM antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
EKRHTRDSEAQR
Sequence Length
288
Applicable Applications for anti-STOM antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Liver shows membrane staining of hepatocytes and of sinusoidal lining cells. Recommended concentration, 5-10ug/ml.
Western Blot: Approx 30kDa band observed in Human Erythrocytes, Spleen, Bone Marrow and Lung lysates (calculated MW of 31.7kDa according to NP_004090.4). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence EKRHTRDSEAQR-C, from the N Terminus of the protein sequence according to NP_004090.4; NP_937837.1.
Epitope
N Terminus
Note
This antibody is expected to recognize both reported isoforms (NP_004090.4; NP_937837.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

((5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

Immunohistochemistry (IHC) ((5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,475 Da
NCBI Official Full Name
erythrocyte band 7 integral membrane protein isoform a
NCBI Official Synonym Full Names
stomatin
NCBI Official Symbol
STOM
NCBI Official Synonym Symbols
BND7; EPB7; EPB72
NCBI Protein Information
erythrocyte band 7 integral membrane protein
UniProt Protein Name
Erythrocyte band 7 integral membrane protein
Protein Family
UniProt Gene Name
STOM
UniProt Synonym Gene Names
BND7; EPB72
UniProt Entry Name
STOM_HUMAN

NCBI Description

This gene encodes a member of a highly conserved family of integral membrane proteins. The encoded protein localizes to the cell membrane of red blood cells and other cell types, where it may regulate ion channels and transporters. Loss of localization of the encoded protein is associated with hereditary stomatocytosis, a form of hemolytic anemia. There is a pseudogene for this gene on chromosome 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]

Uniprot Description

stomatin: Thought to regulate cation conductance. May regulate ASIC2 and ASIC3 gating. Belongs to the band 7/mec-2 family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: cytoplasm; cytoskeleton; endoplasmic reticulum; extracellular space; integral to plasma membrane; lipid raft; melanosome; membrane; mitochondrion; perinuclear region of cytoplasm; vesicle

Molecular Function: protein binding; protein homodimerization activity

Biological Process: positive regulation of defense response to virus by host; protein homooligomerization

Disease: Overhydrated Hereditary Stomatocytosis

Research Articles on STOM

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Product Notes

The STOM stom (Catalog #AAA422940) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Stomatin Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Stomatin can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Liver shows membrane staining of hepatocytes and of sinusoidal lining cells. Recommended concentration, 5-10ug/ml. Western Blot: Approx 30kDa band observed in Human Erythrocytes, Spleen, Bone Marrow and Lung lysates (calculated MW of 31.7kDa according to NP_004090.4). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the STOM stom for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EKRHTRDSEA QR. It is sometimes possible for the material contained within the vial of "Stomatin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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