Human GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) ELISA Kit | GABARAPL2 elisa kit

Human GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) ELISA Kit

Cat. Num. AAA2025203

• Gene Names: GABARAPL2; ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16
• Reactivity: Human
• Synonyms: GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2); Human GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) ELISA Kit; ATG8; GATE16; GEF2; FLC3A; General protein transport factor p16; Ganglioside expression factor 2; Golgi-associated ATPase enhancer of 16 kDa; MAP1 light chain 3-related; GABARAPL2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2).; No significant cross-reactivity or interference between GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) and analogues was observed.
• Sequence Length: 117

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GABARAPL2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GABARAPL2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 13375573
• NCBI GeneID: 11345
• Molecular Weight: 13,667 Da
• NCBI Official Full Name: GABA-A receptor-associated protein like 2
• NCBI Official Synonym Full Names: GABA(A) receptor-associated protein like 2
• NCBI Official Symbol: GABARAPL2
• NCBI Official Synonym Symbols: ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16
• NCBI Protein Information: gamma-aminobutyric acid receptor-associated protein-like 2
• UniProt Protein Name: Gamma-aminobutyric acid receptor-associated protein-like 2
• Protein Family: Gamma-aminobutyric acid receptor-associated protein
• UniProt Gene Name: GABARAPL2
• UniProt Synonym Gene Names: FLC3A; GEF2; GEF-2; GATE-16
• UniProt Entry Name: GBRL2_HUMAN

Uniprot Description

GABARAPL2: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Implicated in intra-Golgi transport and post-mitotic Golgi re-assembly. Modulates the activity of SNAREs in the Golgi apparatus and is therefore an essential component of intra-Golgi transport and post-mitotic Golgi re-assembly. It first stimulates the ATPase activity of NSF, which in turn stimulates the association with GOSR1. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ULK1. Phosphorylated upon DNA damage, probably by ATM or ATR.

Protein type: Adaptor/scaffold; Autophagy; Vesicle; Ubiquitin-like modifier; Microtubule-binding

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: Golgi membrane; Golgi apparatus; extrinsic to membrane; cytoplasm; autophagic vacuole; cytoplasmic vesicle; intracellular; cytosol

Molecular Function: SNARE binding; protein binding; microtubule binding; beta-tubulin binding; GABA receptor binding; ATPase binding

Biological Process: intra-Golgi vesicle-mediated transport; protein transport; positive regulation of ATPase activity; mitochondrion degradation; autophagy; autophagic vacuole formation; cellular response to nitrogen starvation