Rabbit anti-Human GABARAPL2 Polyclonal Antibody | anti-GABARAPL2 antibody

Anti-GABARAPL2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8107404

• Gene Names: GABARAPL2; ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: GABARAPL2; Polyclonal Antibody; Anti-GABARAPL2 Antibody; Rabbit Polyclonal; GATE-16/GABARAPL2 Antibody; Rabbit PAb; Antigen Affinity Purified; GABA(A) receptor-associated protein-like 2; Anti-ATG8 Antibody; Anti-ATG8C Antibody; Anti-GATE-16 Antibody; Anti-GATE16 Antibody; Anti-GEF-2 Antibody; Anti-GEF2 Antibody; anti-GABARAPL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human GATE-16/GABARAPL2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GABARAPL2 antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:5000-1:10,000; IHC-P: 1:1000-1:4000
• Immunogen: Recombinant Human GATE-16/GABARAPL2 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human GATE-16/GABARAPL2 (rh GATE-16/GABARAPL2). GATE-16/GABARAPL2 specific IgG was purified by Human GATE-16/GABARAPL2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human GABARAPL2 in human prostate with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human GABARAPL2 in human brain with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-GABARAPL2 antibody

GATE-16, also known as ATG8, belongs to the MAP1 LC3 family. It is expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. GATE-16 is expressed at very low levels in lung, thymus and small intestine. GATE-16 is involved in intra-Golgi traffic. It modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1.

References

Ewing Rob M, et al. (2007) Large-scale mapping of human protein-protein interactions by mass spectrometry. Mol Syst Biol. 3(1):89. Okazaki N, et al. (2000) Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation. Brain Res Mol Brain Res. 85(1-2):1-12. Xin Y, et al. (2001) Cloning, expression patterns, and chromosome localization of three human and two mouse homologues of GABA(A) receptor-associated protein. Genomics. 74 (3):408-13.

NCBI and Uniprot Product Information

• NCBI GI #: 6005768
• NCBI GeneID: 11345
• NCBI Accession #: NP_009216.1
• NCBI GenBank Nucleotide #: NM_007285.6
• UniProt Accession #: P60520; O08765; Q6FG91; Q9DCP8; Q9UQF7
• Molecular Weight: 13,667 Da
• NCBI Official Full Name: gamma-aminobutyric acid receptor-associated protein-like 2
• NCBI Official Synonym Full Names: GABA(A) receptor-associated protein-like 2
• NCBI Official Symbol: GABARAPL2
• NCBI Official Synonym Symbols: ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16
• NCBI Protein Information: gamma-aminobutyric acid receptor-associated protein-like 2; MAP1 light chain 3 related protein; MAP1 light chain 3-related protein; ganglioside expression factor 2; general protein transport factor p16; golgi-associated ATPase enhancer of 16 kDa
• UniProt Protein Name: Gamma-aminobutyric acid receptor-associated protein-like 2
• Protein Family: Gamma-aminobutyric acid receptor-associated protein
• UniProt Gene Name: GABARAPL2
• UniProt Synonym Gene Names: FLC3A; GEF2; GEF-2; GATE-16
• UniProt Entry Name: GBRL2_HUMAN

Uniprot Description

GABARAPL2: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Implicated in intra-Golgi transport and post-mitotic Golgi re-assembly. Modulates the activity of SNAREs in the Golgi apparatus and is therefore an essential component of intra-Golgi transport and post-mitotic Golgi re-assembly. It first stimulates the ATPase activity of NSF, which in turn stimulates the association with GOSR1. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ULK1. Phosphorylated upon DNA damage, probably by ATM or ATR.

Protein type: Microtubule-binding; Autophagy; Vesicle; Adaptor/scaffold; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: Golgi membrane; Golgi apparatus; extrinsic to membrane; cytoplasm; autophagic vacuole; intracellular; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; SNARE binding; microtubule binding; beta-tubulin binding; GABA receptor binding; ATPase binding

Biological Process: intra-Golgi vesicle-mediated transport; protein transport; positive regulation of ATPase activity; mitochondrion degradation; autophagy; autophagic vacuole formation; cellular response to nitrogen starvation