Human Steroidogenic Acute Regulatory Protein (STAR) ELISA Kit | STAR elisa kit

Human Steroidogenic Acute Regulatory Protein (STAR) ELISA Kit

Cat. Num. AAA2024407

• Gene Names: STAR; STARD1
• Reactivity: Human
• Synonyms: Steroidogenic Acute Regulatory Protein (STAR); Human Steroidogenic Acute Regulatory Protein (STAR) ELISA Kit; STARD1; START domain-containing protein 1; STAR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Steroidogenic Acute Regulatory Protein (STAR).; No significant cross-reactivity or interference between Steroidogenic Acute Regulatory Protein (STAR) and analogues was observed.
• Sequence Length: 285

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.064ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Steroidogenic Acute Regulatory Protein (STAR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Steroidogenic Acute Regulatory Protein (STAR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STAR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Steroidogenic Acute Regulatory Protein (STAR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Steroidogenic Acute Regulatory Protein (STAR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Steroidogenic Acute Regulatory Protein (STAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Steroidogenic Acute Regulatory Protein (STAR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for STAR elisa kit

Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 2661026
• NCBI GeneID: 6770
• UniProt Accession #: P49675; Q16396
• Molecular Weight: 31,914 Da
• NCBI Official Full Name: steroidogenic acute regulatory protein
• NCBI Official Synonym Full Names: steroidogenic acute regulatory protein
• NCBI Official Symbol: STAR
• NCBI Official Synonym Symbols: STARD1
• NCBI Protein Information: steroidogenic acute regulatory protein, mitochondrial
• UniProt Protein Name: Steroidogenic acute regulatory protein, mitochondrial
• Protein Family: Steroidogenic acute regulatory protein
• UniProt Gene Name: STAR
• UniProt Synonym Gene Names: STARD1; StAR; StARD1
• UniProt Entry Name: STAR_HUMAN

NCBI Description

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq, Jul 2008]

Uniprot Description

STAR: Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone. May interact with TSPO. Expressed in gonads, adrenal cortex and kidney.

Protein type: Carrier; Lipid-binding; Mitochondrial

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: mitochondrial crista; neuron projection; cell soma; mitochondrial intermembrane space; cytosol

Molecular Function: cholesterol transporter activity; cholesterol binding

Biological Process: steroid metabolic process; response to nicotine; diterpenoid metabolic process; dibenzo-p-dioxin metabolic process; regulation of steroid biosynthetic process; estrogen biosynthetic process; biphenyl metabolic process; circadian sleep/wake cycle, REM sleep; negative regulation of neuron apoptosis; response to nutrient; response to corticosterone stimulus; response to drug; bile acid biosynthetic process; cholesterol metabolic process; insecticide metabolic process; positive regulation of neurogenesis; progesterone biosynthetic process; fractalkine metabolic process; phthalate metabolic process; male gonad development; intracellular cholesterol transport; response to herbicide; glucocorticoid metabolic process; response to ethanol; response to hydrogen peroxide; cellular response to insulin stimulus; response to estrogen stimulus; response to lead ion; C21-steroid hormone biosynthetic process; response to ionizing radiation; brain development; response to activity; steroid biosynthetic process; regulation of neuronal synaptic plasticity; phenol metabolic process

Disease: Lipoid Congenital Adrenal Hyperplasia