Porcine Steroidogenic acute regulatory protein ELISA Kit | StAR elisa kit

Porcine Steroidogenic acute regulatory protein ELISA Kit

Cat. Num. AAA746861

• Gene Names: STAR; STARD1
• Reactivity: Porcine
• Synonyms: Steroidogenic acute regulatory protein; Porcine Steroidogenic acute regulatory protein ELISA Kit; StAR elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This STAR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine STAR. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This STAR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine STAR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for StAR elisa kit

Principle of the Assay: STAR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-STAR antibody and an STAR-HRP conjugate. The assay sample and buffer are incubated together with STAR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STAR concentration since STAR from samples and STAR-HRP conjugate compete for the anti-STAR antibody binding site. Since the number of sites is limited, as more sites are occupied by STAR from the sample, fewer sites are left to bind STAR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STAR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for StAR elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 2661026
• NCBI GeneID: 6770
• Molecular Weight: 31,914 Da
• NCBI Official Full Name: steroidogenic acute regulatory protein
• NCBI Official Synonym Full Names: steroidogenic acute regulatory protein
• NCBI Official Symbol: STAR
• NCBI Official Synonym Symbols: STARD1
• NCBI Protein Information: steroidogenic acute regulatory protein, mitochondrial; cholesterol trafficker; START domain containing 1; steroid acute regulatory protein; START domain-containing protein 1; mitochondrial steroid acute regulatory protein; StAR-related lipid transfer (START) domain containing 1
• UniProt Protein Name: Steroidogenic acute regulatory protein, mitochondrial
• Protein Family: Steroidogenic acute regulatory protein
• UniProt Gene Name: STAR
• UniProt Synonym Gene Names: STARD1; StAR; StARD1
• UniProt Entry Name: STAR_HUMAN

NCBI Description

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq, Jul 2008]

Uniprot Description

STAR: Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone. May interact with TSPO. Expressed in gonads, adrenal cortex and kidney.

Protein type: Carrier; Mitochondrial; Lipid-binding

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: mitochondrial crista; neuron projection; cell soma; mitochondrial intermembrane space; cytosol

Molecular Function: cholesterol transporter activity; cholesterol binding

Biological Process: steroid metabolic process; response to nicotine; diterpenoid metabolic process; dibenzo-p-dioxin metabolic process; regulation of steroid biosynthetic process; estrogen biosynthetic process; biphenyl metabolic process; circadian sleep/wake cycle, REM sleep; negative regulation of neuron apoptosis; response to nutrient; response to corticosterone stimulus; response to drug; bile acid biosynthetic process; cholesterol metabolic process; progesterone biosynthetic process; positive regulation of neurogenesis; insecticide metabolic process; fractalkine metabolic process; male gonad development; phthalate metabolic process; intracellular cholesterol transport; response to herbicide; glucocorticoid metabolic process; response to ethanol; cellular response to insulin stimulus; response to hydrogen peroxide; response to estrogen stimulus; response to lead ion; C21-steroid hormone biosynthetic process; response to ionizing radiation; response to activity; brain development; steroid biosynthetic process; phenol metabolic process; regulation of neuronal synaptic plasticity

Disease: Lipoid Congenital Adrenal Hyperplasia