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Typical Testing Data/Standard Curve (for reference only)

Rat Corticotropin Releasing Hormone Receptor 1 (CRHR1) ELISA Kit | CRHR1 elisa kit

Rat Corticotropin Releasing Hormone Receptor 1 (CRHR1) ELISA Kit

Gene Names
CRHR1; CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRF-R-1; CRH-R-1
Reactivity
Rat
Synonyms
Corticotropin Releasing Hormone Receptor 1 (CRHR1); Rat Corticotropin Releasing Hormone Receptor 1 (CRHR1) ELISA Kit; CRF-R; CRF1; CRFR1; CRH-R1h; CRHR; CRHR1f; Corticotropin-releasing factor receptor 1; CRHR1 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Corticotropin Releasing Hormone Receptor 1 (CRHR1).
No significant cross-reactivity or interference between Corticotropin Releasing Hormone Receptor 1 (CRHR1) and analogues was observed.
Sequence Length
240
Assay Type
Double-antibody Sandwich
Samples
Tissue Homogenates, Cell Lysates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.054ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Corticotropin Releasing Hormone Receptor 1 (CRHR1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Corticotropin Releasing Hormone Receptor 1 (CRHR1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CRHR1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Corticotropin Releasing Hormone Receptor 1 (CRHR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Corticotropin Releasing Hormone Receptor 1 (CRHR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Corticotropin Releasing Hormone Receptor 1 (CRHR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Corticotropin Releasing Hormone Receptor 1 (CRHR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
28,122 Da
NCBI Official Full Name
corticotropin releasing hormone receptor 1
NCBI Official Synonym Full Names
corticotropin releasing hormone receptor 1
NCBI Official Symbol
CRHR1
NCBI Official Synonym Symbols
CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRF-R-1; CRH-R-1
NCBI Protein Information
corticotropin-releasing factor receptor 1
UniProt Protein Name
Corticotropin-releasing factor receptor 1
UniProt Gene Name
CRHR1
UniProt Synonym Gene Names
CRFR; CRFR1; CRHR; CRF-R-1; CRF-R1; CRFR-1; CRH-R-1; CRH-R1

NCBI Description

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants. Naturally-occurring readthrough transcription between this gene and upstream GeneID:147081 results in transcripts that encode isoforms that share similarity with the products of this gene. [provided by RefSeq, Aug 2016]

Uniprot Description

G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). Has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. Inhibits the activity of the calcium channel CACNA1H. Required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress. Plays a role in the response to anxiogenic stimuli.

Research Articles on CRHR1

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Product Notes

The Rat CRHR1 crhr1 (Catalog #AAA2704803) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2704803 ELISA Kit recognizes Rat CRHR1. It is sometimes possible for the material contained within the vial of "Corticotropin Releasing Hormone Receptor 1 (CRHR1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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