Collagen Type III Alpha 1 (COL3a1) Recombinant Protein | COL3a1 recombinant protein

Recombinant Collagen Type III Alpha 1 (COL3a1)

Cat. Num. AAA2011212

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Collagen Type III Alpha 1 (COL3a1); Recombinant Collagen Type III Alpha 1 (COL3a1); COL3a1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEFELRRQAC-RGSEGSPGHP GQPGPPGPPG APGPCCGGGA AIAGVGGEKS GGFSPYYGDD PMDFKINTEE IMSSLKSVNG QIESLISPDG SRKNPARNCR DLKFCHPELK SGEYWVDPNQ GCKMDAIKVF CNMETGETCI NASPMTVPRK HWWTDAGAEK KHVWFGESMN GGFQFSYGNP DLPEDVLDVQ LAFLRLLSSR ASQNITYHCK NSIAYMDQAN GNVKKSLKLM GSNEGEFKAE GNSKFTYTVL EDGCTKHTGE WSKTVFEYQT RKAMR
• Sequence Length: 1463

• Applicable Applications for COL3a1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Arg1171~Arg1435 (Accession # P13941) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 56711254
• NCBI GeneID: 84032
• NCBI Accession #: NP_114474.1
• NCBI GenBank Nucleotide #: NM_032085.1
• UniProt Accession #: P13941; O70604; Q5PQT6
• Molecular Weight: 31.2kDa
• NCBI Official Full Name: collagen alpha-1(III) chain
• NCBI Official Synonym Full Names: collagen, type III, alpha 1
• NCBI Official Symbol: Col3a1
• NCBI Protein Information: collagen alpha-1(III) chain; collagen alpha-1(III) chain; procollagen, type III, alpha 1
• UniProt Protein Name: Collagen alpha-1(III) chain
• Protein Family: Collagen
• UniProt Gene Name: Col3a1
• UniProt Entry Name: CO3A1_RAT

NCBI Description

may be involved in craniofacial development [RGD, Feb 2006]

Uniprot Description

CO3A1: Collagen type III occurs in most soft connective tissues along with type I collagen. Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3); also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity. Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas. Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA). AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells. Belongs to the fibrillar collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Extracellular matrix; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; collagen; collagen type III

Molecular Function: integrin binding; metal ion binding; platelet-derived growth factor binding; extracellular matrix structural constituent; SMAD binding

Biological Process: skin development; integrin-mediated signaling pathway; blood vessel development; collagen fibril organization; wound healing; cell-matrix adhesion; heart development; negative regulation of immune response; positive regulation of Rho protein signal transduction; response to radiation; gut development; response to mechanical stimulus; transforming growth factor beta receptor signaling pathway; response to cytokine stimulus; fibril organization and biogenesis; cerebral cortex development; peptide cross-linking; skeletal development; aging