Rabbit anti-Human DOK2 Polyclonal Antibody | anti-DOK2 antibody

DOK2, CT (Docking Protein 2, Downstream Of Tyrosine Kinase 2, p56(dok-2)) (PE)

Cat. Num. AAA6472833

• Gene Names: DOK2; p56DOK; p56dok-2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: DOK2; Polyclonal Antibody; CT (Docking Protein 2; Downstream Of Tyrosine Kinase 2; p56(dok-2)) (PE); anti-DOK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DOK2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-DOK2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human DOK2 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-DOK2 Pab in 174xCEM cell lysate. DOK2 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB)

(Western blot analysis of DOK2 (arrow) using DOK2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DOK2 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-DOK2 antibody

Antibodies; Signal Transduction

References

1. Salomon, A.R., et al., Proc. Natl. Acad. Sci. USA 100(2):443-448 (2003). 2. Di Cristofano, A., et al., J. Biol. Chem. 273(9):4827-4830 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 41406050
• NCBI GeneID: 9046
• NCBI Accession #: NP_003965.2
• NCBI GenBank Nucleotide #: NM_003974.2
• UniProt Accession #: O60496; Q8N5A4
• Molecular Weight: 45,379 Da
• NCBI Official Full Name: docking protein 2
• NCBI Official Synonym Full Names: docking protein 2, 56kDa
• NCBI Official Symbol: DOK2
• NCBI Official Synonym Symbols: p56DOK; p56dok-2
• NCBI Protein Information: docking protein 2; p56(dok-2); downstream of tyrosine kinase 2
• UniProt Protein Name: Docking protein 2
• Protein Family: Docking protein
• UniProt Gene Name: DOK2
• UniProt Entry Name: DOK2_HUMAN

NCBI Description

The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq, Jul 2008]

Uniprot Description

DOK2: a member of the p62dok family of proteins that interact with receptor tyrosine kinases and mediate particular biological responses. Constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/Abl), a chimeric protein whose presence is associated with CML. Contains a pleckstrin homology domain at the amino terminus, 13 potential tyrosine phosphorylation sites, and six PXXP SH3 recognition motifs. Binds p120 (RasGAP) from CML cells.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: cytosol

Molecular Function: receptor signaling protein activity; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding

Biological Process: cell surface receptor linked signal transduction; Ras protein signal transduction; blood coagulation; signal transduction; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway