Rat Collagen Type III Alpha 1 (COL3a1) RTU ELISA Kit | COL3a1 rtu elisa kit

Rat Collagen Type III Alpha 1 (COL3a1) ELISA Kit

Cat. Num. AAA458476

• Reactivity: Rat
• Synonyms: Collagen Type III Alpha 1 (COL3a1); Rat Collagen Type III Alpha 1 (COL3a1) ELISA Kit; COL3-A1; COL3A1; EDS4A; Collagen Type III Alpha 1; Ehlers-Danlos Syndrome Type IV Autosomal Dominant; Collagen Alpha-1(III)chain; COL3a1 rtu elisa kit

• Reactivity: Rat
• Sequence Length: 1463

• Assay Type: Sandwich
• Samples: Serum, Plasma or Other Biological Fluids.
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.277ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL3a1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL3a1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to COL3a1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL3a1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of COL3a1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 56711254
• NCBI GeneID: 84032
• NCBI Accession #: NP_114474.1
• NCBI GenBank Nucleotide #: NM_032085.1
• UniProt Accession #: P13941; O70604; Q5PQT6
• Molecular Weight: 138,936 Da
• NCBI Official Full Name: collagen alpha-1(III) chain
• NCBI Official Synonym Full Names: collagen type III alpha 1 chain
• NCBI Official Symbol: Col3a1
• NCBI Protein Information: collagen alpha-1(III) chain
• UniProt Protein Name: Collagen alpha-1(III) chain
• Protein Family: Collagen
• UniProt Gene Name: Col3a1

NCBI Description

may be involved in craniofacial development [RGD, Feb 2006]

Uniprot Description

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12 ().