Alpha-2-Macroglobulin (a2M) Recombinant Protein | a2M recombinant protein

Recombinant Alpha-2-Macroglobulin (a2M)

Cat. Num. AAA2010061

• Gene Names: A2m; Mam; A2m1; A2maa; A2MAC1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Alpha-2-Macroglobulin (a2M); Recombinant Alpha-2-Macroglobulin (a2M); a2M recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- VFRPNSG LPATRALLSV MASPQSLCGL RAVDQSVLLM KPETELSASL IYDLLPVKDL TGFPQGADQR EEDTNGCVKQ NDTYINGILY SPVQNTNEED MYGFLKDMGL KVFTNSNIRK PKVCERLRDN KGIPAAYHLV SQSHMDAFLE SSESPTETRR SYFP
• Sequence Length: 1472

• Applicable Applications for a2M recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Val574~Pro734 (Accession # P06238) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 158138551
• NCBI GeneID: 24153
• NCBI Accession #: NP_036620.2
• NCBI GenBank Nucleotide #: NM_012488.2
• UniProt Accession #: P06238; Q4FZY3
• Molecular Weight: 19.5kDa
• NCBI Official Full Name: alpha-2-macroglobulin
• NCBI Official Synonym Full Names: alpha-2-macroglobulin
• NCBI Official Symbol: A2m
• NCBI Official Synonym Symbols: Mam; A2m1; A2maa; A2MAC1
• NCBI Protein Information: alpha-2-macroglobulin; alpha-2-M; alpha-2-macroglobulin-P
• UniProt Protein Name: Alpha-2-macroglobulin
• Protein Family: Alpha-2-macroglobulin
• UniProt Gene Name: A2m
• UniProt Synonym Gene Names: A2m1; Alpha-2-M
• UniProt Entry Name: A2MG_RAT

NCBI Description

plays a role in acute phase response; activated form induces a decrease in N-methyl-D-aspartate (NMDA) mediated calcium signaling [RGD, Feb 2006]

Uniprot Description

Function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Subunit structure: Homotetramer; disulfide-linked.

Subcellular location: Secreted.

Tissue specificity: Highest constitutive expression in ovary. Low level in testis, uterus and non-acute phase liver. Protein found in plasma. Ref.5

Induction: By inflammatory stimulus in liver. The level of this protein increases during acute phase, then decreases again. Ref.1

Sequence similarities: Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. [View classification]