Typical Testing Data/Standard Curve (for reference only)
Rat alpha-2-macroglobulin ELISA Kit | A2M elisa kit
Rat alpha2 macroglobulin, alpha2 MG ELISA Kit
Gene Names
A2m; Mam; A2m1; A2maa; A2MAC1
Reactivity
Rat
Synonyms
alpha-2-macroglobulin; Rat alpha2 macroglobulin; alpha2 MG ELISA Kit; CPAMD5; DKFZp779B086; FWP007; S863-7; ; A2M elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat alpha2 MG. No significant cross-reactivity or interference between rat alpha2 MG and analogues was observed.
Sequence Length
1472
Samples
Serum, plasma
Assay Type
Quantitative Competitive
Detection Range
3.125 ng/ml-800 ng/ml
Sensitivity
<3.125 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for A2M elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rat alpha2 MG. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for rat alpha2 MG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of rat alpha2 MG in samples. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
163,785 Da
NCBI Official Full Name
alpha-2-macroglobulin
NCBI Official Synonym Full Names
alpha-2-macroglobulin
NCBI Official Symbol
A2m
NCBI Official Synonym Symbols
Mam; A2m1; A2maa; A2MAC1
NCBI Protein Information
alpha-2-macroglobulin; alpha-2-M; alpha-2-macroglobulin-P
UniProt Protein Name
Alpha-2-macroglobulin
Protein Family
UniProt Gene Name
A2m
UniProt Synonym Gene Names
A2m1; Alpha-2-M
UniProt Entry Name
A2MG_RAT
NCBI Description
plays a role in acute phase response; activated form induces a decrease in N-methyl-D-aspartate (NMDA) mediated calcium signaling [RGD, Feb 2006]
Uniprot Description
Function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Research Articles on A2M
Similar Products
Product Notes
The Rat A2M a2m (Catalog #AAA702834) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA702834 ELISA Kit recognizes Rat A2M. It is sometimes possible for the material contained within the vial of "alpha-2-macroglobulin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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