Rabbit anti-Human Aspartate Aminotransferase (AST) Polyclonal Antibody | anti-AST antibody

Polyclonal Antibody to Aspartate Aminotransferase (AST)

Cat. Num. AAA2007171

• Gene Names: GOT1; AST1; cCAT; GIG18; cAspAT; ASTQTL1
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Aspartate Aminotransferase (AST); Polyclonal Antibody; Polyclonal Antibody to Aspartate Aminotransferase (AST); anti-AST antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against AST. It has been selected for its ability to recognize AST in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 0.5mg/ml (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF-MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFLARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ
• Sequence Length: 448

• Applicable Applications for anti-AST antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant AST (Met1~Gln413) expressed in E.coli.
• Cross Reactivity: Human, Mouse, Rat
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2052584
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Heart Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant AST, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Kidney Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Cancer Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 4504067
• NCBI GeneID: 2805
• NCBI Accession #: NP_002070.1
• NCBI GenBank Nucleotide #: NM_002079.2
• UniProt Accession #: P17174; Q5VW80; B2R6R7; B7Z7E9
• Molecular Weight: 44,147 Da
• NCBI Official Full Name: aspartate aminotransferase, cytoplasmic
• NCBI Official Synonym Full Names: glutamic-oxaloacetic transaminase 1
• NCBI Official Symbol: GOT1
• NCBI Official Synonym Symbols: AST1; cCAT; GIG18; cAspAT; ASTQTL1
• NCBI Protein Information: aspartate aminotransferase, cytoplasmic
• UniProt Protein Name: Aspartate aminotransferase, cytoplasmic
• Protein Family: Allatostatin
• UniProt Gene Name: GOT1
• UniProt Synonym Gene Names: cAspAT; cCAT

NCBI Description

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]

Uniprot Description

GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; EC 2.6.1.3; Transferase

Chromosomal Location of Human Ortholog: 10q24.2

Cellular Component: axon terminus; cytoplasm; cytosol; extracellular exosome; lysosome; mitochondrion; nucleoplasm; nucleus

Molecular Function: carboxylic acid binding; L-aspartate:2-oxoglutarate aminotransferase activity; L-cysteine:2-oxoglutarate aminotransferase activity; phosphatidylserine decarboxylase activity; pyridoxal phosphate binding

Biological Process: 2-oxoglutarate metabolic process; amino acid biosynthetic process; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; cellular response to insulin stimulus; fatty acid homeostasis; gluconeogenesis; glutamate catabolic process to 2-oxoglutarate; glutamate catabolic process to aspartate; glutamate metabolic process; glycerol biosynthetic process; Notch signaling pathway; oxaloacetate metabolic process; response to glucocorticoid stimulus

Disease: Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1