Rabbit anti-Mouse High Mobility Group Protein 1 (HMG1) Polyclonal Antibody | anti-HMG1 antibody

Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)

Cat. Num. AAA2007018

• Gene Names: Hmgb1; p30; Hmg1; HMG-1; SBP-1
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: High Mobility Group Protein 1 (HMG1); Polyclonal Antibody; Polyclonal Antibody to High Mobility Group Protein 1 (HMG1); anti-HMG1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against HMG1. It has been selected for its ability to recognize HMG1 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF-MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE

• Applicable Applications for anti-HMG1 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant HMG1 (Met1~Glu215) expressed in E.coli.
• Cross Reactivity: Human, Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2041950
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Kidney lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse HMG1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))

Western Blot (WB)

(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse HMG1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))

Western Blot (WB)

(Western Blot: Sample: Human HepG2 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse HMG1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))

Knockout Validation

(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate;;Lane 2: HMG1 knockout HepG2 cell lysate;;Predicted MW: 25kDa ;Observed MW: 30kDa;Primary Ab: 1ug/ml Rabbit Anti-Mouse HMG1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))

Product Categories/Family for anti-HMG1 antibody

Knock-Out Validation Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 926657659
• NCBI GeneID: 15289
• NCBI Accession #: NP_001300823.1
• NCBI GenBank Nucleotide #: NM_001313894.1
• UniProt Accession #: P63158; P07155; P27109; P27428
• Molecular Weight: 24,894 Da
• NCBI Official Full Name: high mobility group protein B1
• NCBI Official Synonym Full Names: high mobility group box 1
• NCBI Official Symbol: Hmgb1
• NCBI Official Synonym Symbols: p30; Hmg1; HMG-1; SBP-1
• NCBI Protein Information: high mobility group protein B1
• UniProt Protein Name: High mobility group protein B1
• Protein Family: High mobility group protein
• UniProt Gene Name: Hmgb1
• UniProt Synonym Gene Names: Hmg-1; Hmg1; HMG-1

NCBI Description

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

Uniprot Description

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family.

Protein type: DNA repair, damage; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5 G3|5 89.18 cM

Cellular Component: cytoplasm; early endosome; extracellular region; extracellular space; neuron projection; nucleus

Molecular Function: bent DNA binding; calcium-dependent protein kinase regulator activity; crossed form four-way junction DNA binding; cytokine activity; double-stranded DNA binding; double-stranded RNA binding; open form four-way junction DNA binding; phosphatidylserine binding; protein binding; protein kinase activator activity; single-stranded RNA binding

Biological Process: base-excision repair; chromatin assembly; DNA geometric change; endothelial cell proliferation; eye development; induction of positive chemotaxis; inflammatory response; lung development; macrophage activation involved in immune response; myeloid dendritic cell activation; negative regulation of apoptotic cell clearance; plasmacytoid dendritic cell activation; positive regulation of cell migration; positive regulation of ERK1 and ERK2 cascade; positive regulation of glycogen catabolic process; positive regulation of innate immune response; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-6 production; positive regulation of mesenchymal cell proliferation; positive regulation of mitotic cell cycle; positive regulation of myeloid cell differentiation; positive regulation of NIK/NF-kappaB signaling; positive regulation of protein phosphorylation; positive regulation of toll-like receptor 2 signaling pathway; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; positive regulation of wound healing; regulation of autophagy; regulation of nucleotide-excision repair; regulation of T cell mediated immune response to tumor cell; regulation of tolerance induction; response to glucocorticoid stimulus