Testing Data
(mmunofluorescence staining of PFA fixed permeablized NIH-3T3 cells with Hamster anti Mouse high mobility group protein B1, signal was visualized using Goat anti Hamster IgG:biotin followed by streptavidin:)
Hamster High Mobility Group Protein B1 Monoclonal Antibody | anti-HMGB1 antibody
High Mobility Group Protein B1 (HMGB1) (MaxLight 490)
Testing Data
(mmunofluorescence staining of PFA fixed permeablized NIH-3T3 cells with Hamster anti Mouse high mobility group protein B1, signal was visualized using Goat anti Hamster IgG:biotin followed by streptavidin:)
Testing Data
(mmunofluorescence staining of PFA fixed permeablized NIH-3T3 cells with Hamster anti Mouse high mobility group protein B1, signal was visualized using Goat anti Hamster IgG:biotin followed by streptavidin:)
Western Blot (WB)
(Western Blotting analysis of proteins and cell line lysates probed with Hamster anti Mouse high mobility group protein B1)
Western Blot (WB)
(Western Blotting analysis of proteins and cell line lysates probed with Hamster anti Mouse high mobility group protein B1)
Testing Data
(Staining of permeablized RAW264.7 cells with Hamster anti Mouse high mobility group protein B1 followed by Goat anti Hamster IgG:PE)
Testing Data
(Staining of permeablized RAW264.7 cells with Hamster anti Mouse high mobility group protein B1 followed by Goat anti Hamster IgG:PE)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
Uniprot Description
Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed:23519706, PubMed:23446148, PubMed:23994764, PubMed:25048472). Has proangiogenic activity (PubMed:16365390). May be involved in platelet activation. Binds to phosphatidylserine and phosphatidylethanolamide. Bound to RAGE mediates signaling for neuronal outgrowth. May play a role in accumulation of expanded polyglutamine (polyQ) proteins ().
Research Articles on HMGB1
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Product Notes
The HMGB1 hmgb1 (Catalog #AAA6253934) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. The High Mobility Group Protein B1 (HMGB1) (MaxLight 490) reacts with Hamster, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's High Mobility Group Protein B1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). Researchers should empirically determine the suitability of the HMGB1 hmgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "High Mobility Group Protein B1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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