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Western Blot (WB)
(Western blot analysis of Hsp20 on different lysates using anti-Hsp20 antibody at 1/1, 000 dilution. Positive control: Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle)
Rabbit Hsp20 Monoclonal Antibody | anti-Hsp20 antibody
Hsp20 Antibody
Gene Names
HSPB6; Hsp20
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Hsp20; Monoclonal Antibody; Hsp20 Antibody; epididymis luminal protein 55 antibody; FLJ32389 antibody; Heat shock 20 kDa like protein p20 antibody; Heat shock 20 kDa-like protein p20 antibody; Heat shock protein alpha crystallin related B6 antibody; Heat shock protein beta 6 antibody; Heat shock protein beta-6 antibody; Heat shock protein; 20-KD antibody; Heat-shock 27-KD protein 6 antibody; HEL55 antibody; Hsp20 antibody; HspB6 antibody; HSPB6_HUMAN antibody; anti-Hsp20 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SD086-03
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-Hsp20 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC:1:50-1:500
FC/FACS: 1:50-1:100
IHC:1:50-1:500
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of Hsp20 on different lysates using anti-Hsp20 antibody at 1/1, 000 dilution. Positive control: Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle)
Western Blot (WB)
(Western blot analysis of Hsp20 on different lysates using anti-Hsp20 antibody at 1/1, 000 dilution. Positive control: Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Hsp20 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Hsp20 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Hsp20 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Hsp20 antibody. Counter stained with hematoxylin.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with Hsp20 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with Hsp20 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Related Product Information for anti-Hsp20 antibody
The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent poly-peptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27 (Heme Oxygenase 1), HSP 32 and HSP 40. HSP 20 occurs in two complex sizes, dimers and multimers. It is related to stress proteins and occurs most abundantly in skeletal muscle and heart. HSP 20 is considerably shorter at the C-terminus and less polar than other small heat shock proteins.
Product Categories/Family for anti-Hsp20 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,136 Da
NCBI Official Full Name
heat shock protein beta-6
NCBI Official Synonym Full Names
heat shock protein, alpha-crystallin-related, B6
NCBI Official Symbol
HSPB6
NCBI Official Synonym Symbols
Hsp20
NCBI Protein Information
heat shock protein beta-6; heat shock 20 kDa-like protein p20
UniProt Protein Name
Heat shock protein beta-6
Protein Family
UniProt Gene Name
HSPB6
UniProt Synonym Gene Names
HspB6
UniProt Entry Name
HSPB6_HUMAN
NCBI Description
This locus encodes a heat shock protein. The encoded protein likely plays a role in smooth muscle relaxation. [provided by RefSeq, Jan 2012]
Uniprot Description
HSP20: a small heat shock protein of the HSP20 family.
Protein type: Chaperone; Heat shock protein
Chromosomal Location of Human Ortholog: 19q13.12
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding; protein homodimerization activity; structural constituent of eye lens
Biological Process: regulation of muscle contraction
Research Articles on Hsp20
Similar Products
Product Notes
The Hsp20 hspb6 (Catalog #AAA9435958) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hsp20 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Hsp20 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC:1:50-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the Hsp20 hspb6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp20, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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