PHPT1 cdna clone
PHPT1
Synonyms
PHPT1; CGI-202; HEL-S-132P; HSPC141; PHP14; PHPT1 cdna clone
Form/Format
Lyophilized
Sequence
Nucleotide Sequence: ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG CGGACATCTA CGACAAAGTG TCGGGCGACA TGCAGAAGCA AGGCTGCGAC TGTGAGTGTC TGGGCGGCGG GCGCATCTCC CACCAGAGTC AGGACAAGAA GATTCACGTG TACGGCTATT CCATGGCCTA TGGTCCTGCC CAGCACGCCA TTTCAACTGA GAAAATCAAA GCCAAGTACC CCGACTACGA GGTCACCTGG GCTAACGACG GCTACTGA
Translation Sequence: MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
Translation Sequence: MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
Sequence Length
125
Species
Human
Chromosome Location
9q34.3
OMIM Reference Number
610167
cDNA Size
378bp
Vector Description
This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
Vector
(puc19-derived cloning vector)
Preparation Before Usage
1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
Preparation and Storage
Store the plasmid at -20 degree C.
Related Product Information for PHPT1 cdna clone
PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides.
Product Categories/Family for PHPT1 cdna clone
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
14 kDa phosphohistidine phosphatase isoform 3
UniProt Protein Name
14 kDa phosphohistidine phosphatase
Protein Family
UniProt Gene Name
PHPT1
UniProt Synonym Gene Names
PHP14
UniProt Entry Name
PHP14_HUMAN
Uniprot Description
PHPT1: a phosphohistidine phosphatase. Present preferentially in heart and skeletal muscle. Insensitive to inhibition by okadaic acid and EDTA.
Protein type: Carbohydrate Metabolism - fructose and mannose; Phosphatase; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.-; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - thiamine
Chromosomal Location of Human Ortholog: 9q34.3
Cellular Component: cytosol
Molecular Function: phosphohistidine phosphatase activity; calcium channel inhibitor activity; phosphoprotein phosphatase activity
Biological Process: negative regulation of lyase activity; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation
Similar Products
Product Notes
The PHPT1 phpt1 (Catalog #AAA200611) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Nucleotide Sequence: ATGGCGGTGG CGGACCTCGC TCTCATTCCT GATGTGGACA TCGACTCCGA CGGCGTCTTC AAGTATGTGC TGATCCGAGT CCACTCGGCT CCCCGCTCCG GGGCTCCGGC TGCAGAGAGC AAGGAGATCG TGCGCGGCTA CAAGTGGGCT GAGTACCATG CGGACATCTA CGACAAAGTG TCGGGCGACA TGCAGAAGCA AGGCTGCGAC TGTGAGTGTC TGGGCGGCGG GCGCATCTCC CACCAGAGTC AGGACAAGAA GATTCACGTG TACGGCTATT CCATGGCCTA TGGTCCTGCC CAGCACGCCA TTTCAACTGA GAAAATCAAA GCCAAGTACC CCGACTACGA GGTCACCTGG GCTAACGACG GCTACTGA Tran slation Sequence: MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY. It is sometimes possible for the material contained within the vial of "PHPT1, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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