SDS-Page
PHPT1 active protein
PHPT1, 1-125aa, Human, His-tag, E Coli (Bioactivity Validated)
Gene Names
PHPT1; PHP; PHP14; CGI-202; HSPC141; HEL-S-132P
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
PHPT1; 1-125aa; Human; His-tag; E Coli (Bioactivity Validated); 14 kDa phosphohistidine phosphatas; bA216L13.10; CGI-202; DKFZp564M173; HSPC141; PHP14; RP11-216L13.10; PHPT1 active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
Sequence Length
125
Applicable Applications for PHPT1 active protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Biological Activity
Specific activity is >120 units/mg, and is defined as the amount of enzyme that hydrolyze 1nmol f p-nitrophenyl phosphate per minute at pH7.5 at 37C.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for PHPT1 active protein
PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for PHPT1 active protein
References
Srivastava S., et al. (2008) Proc Natl Acad Sci U S A. 105 (38):14442-6.; van Vlierberghe P., et al. (2006) Leukemia. 20 (7):1245-53.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15.9 kDa (145aa), confirmed by MALDI-TOF
NCBI Official Full Name
14 kDa phosphohistidine phosphatase isoform 3
NCBI Official Synonym Full Names
phosphohistidine phosphatase 1
NCBI Official Symbol
PHPT1
NCBI Official Synonym Symbols
PHP; PHP14; CGI-202; HSPC141; HEL-S-132P
NCBI Protein Information
14 kDa phosphohistidine phosphatase
UniProt Protein Name
14 kDa phosphohistidine phosphatase
Protein Family
UniProt Gene Name
PHPT1
UniProt Synonym Gene Names
PHP14; PHP
NCBI Description
This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
Uniprot Description
Exhibits phosphohistidine phosphatase activity.
Research Articles on PHPT1
Similar Products
Product Notes
The PHPT1 phpt1 (Catalog #AAA206566) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's PHPT1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the PHPT1 phpt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY. It is sometimes possible for the material contained within the vial of "PHPT1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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