Rabbit anti-Human, Mouse Pdk2 Polyclonal Antibody | anti-PDK2 antibody

Pdk2, NT (Pdk2, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase isoform 2)

Cat. Num. AAA648984

• Gene Names: PDK2; PDHK2; PDKII
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Pdk2; Polyclonal Antibody; NT (Pdk2; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2; mitochondrial; Pyruvate dehydrogenase kinase isoform 2); Anti -Pdk2; anti-PDK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PDK2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: Mouse Pdk2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-107 amino acids from the N-terminal region of Mouse Pdk2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Pdk2 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Pdk2 antibody detected the Pdk2 protein (arrow).)

Western Blot (WB)

(Western blot analysis of Pdk2 (arrow) using rabbit polyclonal Mouse Pdk2 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Pdk2 gene.)

Western Blot (WB)

(Mouse Pdk2 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Pdk2 antibody detected the Pdk2 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Pdk2 Antibody (N-term) (AP14075a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Pdk2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PDK2 antibody

Pdk2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Product Categories/Family for anti-PDK2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 315630393
• NCBI GeneID: 5164
• NCBI Accession #: NP_001186829.1
• NCBI GenBank Nucleotide #: NM_001199900.1
• UniProt Accession #: Q15119; Q6P515; Q9BS05; A8K3A7; B3KNW0
• Molecular Weight: 46,154 Da
• NCBI Official Full Name: pyruvate dehydrogenase kinase, isozyme 2 isoform 3
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase, isozyme 2
• NCBI Official Symbol: PDK2
• NCBI Official Synonym Symbols: PDHK2; PDKII
• NCBI Protein Information: pyruvate dehydrogenase kinase, isozyme 2; PDH kinase 2; pyruvate dehydrogenase kinase, isoenzyme 2; pyruvate dehydrogenase, lipoamide, kinase isozyme 2, mitochondrial
• UniProt Protein Name: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial
• UniProt Gene Name: PDK2
• UniProt Synonym Gene Names: PDHK2; PDH kinase 2; PDKII
• UniProt Entry Name: PDK2_HUMAN

NCBI Description

This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

Function: Serine/threonine kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis. Ref.1 Ref.6 Ref.10 Ref.11 Ref.12 Ref.13

Catalytic activity: ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate. Ref.1 Ref.8 Ref.9 Ref.11

Enzyme regulation: Activity is enhanced by binding to the pyruvate dehydrogenase subunit DLAT. Inhibited by ADP and pyruvate; these compounds interfere with DLAT binding and thereby inhibit kinase activity. Inhibited by dichloroacetate. Inhibited by AZD7545; this compound interferes with DLAT binding and thereby inhibits kinase activity. Ref.8 Ref.9 Ref.11

Subunit structure: Homodimer, and heterodimer with PDK1. Interacts with the pyruvate dehydrogenase complex subunit DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). Ref.7 Ref.8 Ref.9 Ref.11

Subcellular location: Mitochondrion matrix.

Tissue specificity: Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung. Ref.1

Induction: Down-regulated by insulin. Up-regulated by reactive oxygen species and cigarette smoke extract. Ref.6 Ref.8 Ref.9 Ref.11 Ref.12

Sequence similarities: Belongs to the PDK/BCKDK protein kinase family.Contains 1 histidine kinase domain.