Western Blot (WB)
(Western Blot: Sample: Mouse Serum; Primary Ab: 1ug/ml Rabbit Anti-Mouse AGT Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Angiotensinogen (AGT) Polyclonal Antibody | anti-AGT antibody
Polyclonal Antibody to Angiotensinogen (AGT)
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- AGDRVYIH PFHLLYHNKS TCAQLENPSV ETLPESTFEP VPIQAKTSPV NEKTLHDQLV LAAEKLEDED RKRAAQVAMI ANFVGFRMYK MLNEAGSGAS GAILSPPALF GTLVSFYLGS LDPTASQLQT LLDVPVKEGD CTSRLDGHKV LAALRAVQGL LVTQGGSSSQ TPLLQSIMVG LFTAPGFRLK HSFVQSLALF TPALFPRSLD LSTDPVLATE KINRFIKAVT GWKMNLPLEG VSTDSTLLFN TYVHFQGTMR GFSQLPGVHE FWVDNSISVS VPMISGTGNF QHWSDAQNNF SVTCVPLGER ATLLLIQPHC TSDLDRVEAL IFRNDLLTWI ENPPPRAIRL TLPQLEIRGS YNLQDLLAED KLPTLLGAEA NLSNIGDTNP RVGEVLNSIL LELKAGEEEQ PTTSVQQPGS PEALDVTLSS PFLFAIYEQD SGTLHFLGRV NNPQSVV
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Mouse Serum; Primary Ab: 1ug/ml Rabbit Anti-Mouse AGT Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Serum; Primary Ab: 1ug/ml Rabbit Anti-Mouse AGT Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
NCBI and Uniprot Product Information
Uniprot Description
angiotensin: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1- 8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2. Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT). Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause. Defects in AGT are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Belongs to the serpin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8 E2|8 72.81 cM
Cellular Component: cytoplasm; extracellular space
Molecular Function: angiotensin receptor binding; hormone activity; serine-type endopeptidase inhibitor activity; type 1 angiotensin receptor binding; type 2 angiotensin receptor binding
Biological Process: activation of NF-kappaB-inducing kinase; activation of phospholipase C activity; angiotensin mediated drinking behavior; angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure; artery smooth muscle contraction; astrocyte activation; blood vessel development; brain renin-angiotensin system; branching involved in ureteric bud morphogenesis; cell surface receptor signaling pathway; cell-matrix adhesion; cellular sodium ion homeostasis; cytokine secretion; drinking behavior; elevation of cytosolic calcium ion concentration; establishment of blood-nerve barrier; excretion; extracellular matrix organization; fibroblast proliferation; G-protein coupled receptor signaling pathway; hormone metabolic process; kidney development; negative regulation of angiogenesis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of neuron apoptosis; negative regulation of neurotrophin TRK receptor signaling pathway; negative regulation of tissue remodeling; ovarian follicle rupture; peristalsis; positive regulation of blood pressure; positive regulation of catalytic activity; positive regulation of cell proliferation; positive regulation of cellular protein metabolic process; positive regulation of cytokine secretion; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of fatty acid biosynthetic process; positive regulation of fibroblast proliferation; positive regulation of MAPK cascade; positive regulation of multicellular organism growth; positive regulation of nitric oxide biosynthetic process; positive regulation of organ growth; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of superoxide anion generation; positive regulation of transcription, DNA-templated; regulation of apoptosis; regulation of blood pressure; regulation of calcium ion transport; regulation of gene expression; regulation of heart rate; regulation of inflammatory response; regulation of long-term neuronal synaptic plasticity; regulation of norepinephrine secretion; regulation of renal output by angiotensin; regulation of systemic arterial blood pressure by circulatory renin-angiotensin; regulation of transmission of nerve impulse; renal response to blood flow involved in circulatory renin-angiotensin regulation of systemic arterial blood pressure; renal system process; renin-angiotensin regulation of aldosterone production; response to cold; response to muscle activity involved in regulation of muscle adaptation; response to salt stress; smooth muscle cell differentiation; smooth muscle cell proliferation; stress-activated MAPK cascade; vasoconstriction; vasodilation
Research Articles on AGT
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Product Notes
The AGT agt (Catalog #AAA2002513) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Angiotensinogen (AGT) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Angiotensinogen (AGT) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the AGT agt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- AGDRVYIH PFHLLYHNKS TCAQLENPSV ETLPESTFEP VPIQAKTSPV NEKTLHDQLV LAAEKLEDED RKRAAQVAMI ANFVGFRMYK MLNEAGSGAS GAILSPPALF GTLVSFYLGS LDPTASQLQT LLDVPVKEGD CTSRLDGHKV LAALRAVQGL LVTQGGSSSQ TPLLQSIMVG LFTAPGFRLK HSFVQSLALF TPALFPRSLD LSTDPVLATE KINRFIKAVT GWKMNLPLEG VSTDSTLLFN TYVHFQGTMR GFSQLPGVHE FWVDNSISVS VPMISGTGNF QHWSDAQNNF SVTCVPLGER ATLLLIQPHC TSDLDRVEAL IFRNDLLTWI ENPPPRAIRL TLPQLEIRGS YNLQDLLAED KLPTLLGAEA NLSNIGDTNP RVGEVLNSIL LELKAGEEEQ PTTSVQQPGS PEALDVTLSS PFLFAIYEQD SGTLHFLGRV NNPQSVV. It is sometimes possible for the material contained within the vial of "Angiotensinogen (AGT), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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