Angiotensinogen (AGT) Recombinant Protein | AGT recombinant protein

Recombinant Angiotensinogen (AGT)

Cat. Num. AAA2011101

• Gene Names: Agt; AngI; AngII; Aogen; AI265500; Serpina8
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Angiotensinogen (AGT); Recombinant Angiotensinogen (AGT); AGT recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEFELRRQ-AGDRVYIH PFHLLYHNKS TCAQLENPS VETLPESTFEP VPIQAKTSPV NEKTLHDQLV LAAEKLEDED RKRAAQVAMI ANFVGFRMYK MLNEAGSGAS GAILSPPALF GTLVSFYLGS LDPTASQLQT LLDVPVKEGD CTSRLDGHKV LAALRAVQGL LVTQGGSSSQ TPLLQSIMVG LFTAPGFRLK HSFVQSLALF TPALFPRSLD LSTDPVLATE KINRFIKAVT GWKMNLPLEG VSTDSTLLFN TYVHFQGTMR GFSQLPGVHE FWVDNSISVS VPMISGTGNF QHWSDAQNNF SVTCVPLGER ATLLLIQPHC TSDLDRVEAL IFRNDLLTWI ENPPPRAIRL TLPQLEIRGS YNLQDLLAED KLPTLLGAEA NLSNIGDTNP RVGEVLNSIL LELKAGEEEQ PTTSVQQPGS PEALDVTLSS PFLFAIYEQD SGTLHFLGRV NNPQSVV
• Sequence Length: 477

• Applicable Applications for AGT recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ala23~Val477 (Accession # P11859) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 113881
• NCBI GeneID: 11606
• UniProt Accession #: P11859
• Molecular Weight: 56.1kDa
• NCBI Official Full Name: Angiotensinogen
• NCBI Official Synonym Full Names: angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• NCBI Official Symbol: Agt
• NCBI Official Synonym Symbols: AngI; AngII; Aogen; AI265500; Serpina8
• NCBI Protein Information: angiotensinogen; serpin A8; angiotensin I; angiotensin ll
• UniProt Protein Name: Angiotensinogen
• Protein Family: Angiotensinogen
• UniProt Gene Name: Agt
• UniProt Synonym Gene Names: Serpina8; Ang I; Ang II; Ang III; Ang IV
• UniProt Entry Name: ANGT_MOUSE

Uniprot Description

angiotensin: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1- 8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2. Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT). Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause. Defects in AGT are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cytoplasm

Molecular Function: angiotensin receptor binding; serine-type endopeptidase inhibitor activity; sodium channel regulator activity; hormone activity; type 2 angiotensin receptor binding; type 1 angiotensin receptor binding

Biological Process: positive regulation of catalytic activity; renal system process; extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; establishment of blood-nerve barrier; negative regulation of nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; positive regulation of multicellular organism growth; positive regulation of vasodilation; ovarian follicle rupture; regulation of apoptosis; positive regulation of fibroblast proliferation; regulation of systemic arterial blood pressure by circulatory renin-angiotensin; cell surface receptor linked signal transduction; positive regulation of superoxide release; negative regulation of neuron apoptosis; kidney development; angiotensin mediated regulation of renal output; regulation of calcium ion transport; response to muscle activity involved in regulation of muscle adaptation; regulation of norepinephrine secretion; brain renin-angiotensin system; negative regulation of tissue remodeling; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; phospholipase C activation; angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure; regulation of gene expression; regulation of transmission of nerve impulse; smooth muscle cell differentiation; cytokine secretion; positive regulation of blood pressure; regulation of long-term neuronal synaptic plasticity; peristalsis; cell-matrix adhesion; drinking behavior; positive regulation of cellular protein metabolic process; renin-angiotensin regulation of aldosterone production; smooth muscle cell proliferation; excretion; vasodilation; response to salt stress; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; fibroblast proliferation; positive regulation of epidermal growth factor receptor signaling pathway; regulation of blood pressure; positive regulation of cell proliferation; angiotensin mediated drinking behavior; artery smooth muscle contraction; positive regulation of fatty acid biosynthetic process; blood vessel development; vasoconstriction; cellular sodium ion homeostasis; renal response to blood flow during renin-angiotensin regulation of systemic arterial blood pressure; activation of NF-kappaB-inducing kinase; positive regulation of organ growth; positive regulation of peptidyl-serine phosphorylation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; ureteric bud branching; regulation of inflammatory response; hormone metabolic process; negative regulation of cell growth; response to cold; astrocyte activation