Mouse Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit | FNDC5 elisa kit

Mouse Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit

Cat. Num. AAA2000317

• Gene Names: Fndc5; PeP; Pxp; C87088; AI836596; 1500001L03Rik
• Reactivity: Mouse
• Synonyms: Fibronectin Type III Domain Containing Protein 5 (FNDC5); Mouse Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit; FRCP2; Irisin; •Fibronectin type III repeat-containing protein 2; FNDC5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibronectin Type III Domain Containing Protein 5 (FNDC5).; No significant cross-reactivity or interference between Fibronectin Type III Domain Containing Protein 5 (FNDC5) and analogues was observed.
• Sequence Length: 209

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.5pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibronectin Type III Domain Containing Protein 5 (FNDC5) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibronectin Type III Domain Containing Protein 5 (FNDC5) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FNDC5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibronectin Type III Domain Containing Protein 5 (FNDC5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibronectin Type III Domain Containing Protein 5 (FNDC5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibronectin Type III Domain Containing Protein 5 (FNDC5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibronectin Type III Domain Containing Protein 5 (FNDC5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FNDC5 elisa kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 47059145
• NCBI GeneID: 384061
• NCBI Accession #: NP_081678.1
• NCBI GenBank Nucleotide #: NM_027402.3
• UniProt Accession #: Q8K4Z2; Q9DB97
• Molecular Weight: 23,321 Da
• NCBI Official Full Name: fibronectin type III domain-containing protein 5
• NCBI Official Synonym Full Names: fibronectin type III domain containing 5
• NCBI Official Symbol: Fndc5
• NCBI Official Synonym Symbols: PeP; Pxp; C87088; AI836596; 1500001L03Rik
• NCBI Protein Information: fibronectin type III domain-containing protein 5
• UniProt Protein Name: Fibronectin type III domain-containing protein 5
• Protein Family: Fibronectin type III domain-containing protein
• UniProt Gene Name: Fndc5
• UniProt Synonym Gene Names: Frcp2; Pep; PeP
• UniProt Entry Name: FNDC5_MOUSE

Uniprot Description

FNDC5: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; endoplasmic reticulum; extracellular region; plasma membrane; integral to membrane; peroxisome

Molecular Function: protein binding; hormone activity

Biological Process: response to muscle activity