Typical Testing Data/Standard Curve (for reference only)
Rat Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit | FNDC5 elisa kit
Rat Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit
Gene Names
Fndc5; PeP; Pxp; C87088; AI836596; 1500001L03Rik
Reactivity
Rat
Synonyms
Fibronectin Type III Domain Containing Protein 5 (FNDC5); Rat Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit; FRCP2; Irisin; Fibronectin type III repeat-containing protein 2; FNDC5 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of FMOD. No significant cross-reactivity or interference between FMOD and analogues was observed.
Sequence Length
209
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Human Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
31.2-2,000pg/mL
Sensitivity
13.2pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FMOD were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FMOD were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FNDC5 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FMOD in human serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FMOD. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FMOD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FMOD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FMOD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FMOD. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FMOD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FMOD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FMOD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for FNDC5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,321 Da
NCBI Official Full Name
fibronectin type III domain-containing protein 5 preproprotein
NCBI Official Synonym Full Names
fibronectin type III domain containing 5
NCBI Official Symbol
Fndc5
NCBI Official Synonym Symbols
PeP; Pxp; C87088; AI836596; 1500001L03Rik
NCBI Protein Information
fibronectin type III domain-containing protein 5
UniProt Protein Name
Fibronectin type III domain-containing protein 5
Protein Family
UniProt Gene Name
Fndc5
UniProt Synonym Gene Names
Frcp2; Pep; PeP
NCBI Description
This gene encodes a type I transmembrane protein containing fibronectin type III repeat. The encoded transmembrane protein undergoes proteolytic processing to generate a soluble hormone named irisin that is secreted into the bloodstream. The expression of this gene followed by the secretion of irisin from skeletal muscle is induced by exercise. The ectopic expression of the encoded protein in mice causes an elevation of irisin in blood and improves metabolic health. [provided by RefSeq, Jul 2016]
Uniprot Description
Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA.
Research Articles on FNDC5
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Product Notes
The Rat FNDC5 fndc5 (Catalog #AAA2105007) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2105007 ELISA Kit recognizes Rat FNDC5. Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the FNDC5 fndc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibronectin Type III Domain Containing Protein 5 (FNDC5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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