Human Fibronectin type III domain-containing protein 5 ELISA Kit | FNDC5 elisa kit

Human Fibronectin type III domain-containing protein 5 ELISA Kit

Cat. Num. AAA763823

• Gene Names: Fndc5; PeP; Pxp; C87088; AI836596; 1500001L03Rik
• Reactivity: Human
• Synonyms: Fibronectin type III domain-containing protein 5; Human Fibronectin type III domain-containing protein 5 ELISA Kit; FNDC5/Fibronectin type III domain-containing protein 5/Fibronectin type III repeat-containing protein 2/Irisin/FRCP2; FNDC5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FNDC5. No significant cross-reactivity or interference between FNDC5 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross - reactivity detection between FNDC5 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 209

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78-5000pg/ml
• Sensitivity: < 46.9pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FNDC5were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FNDC5were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Standard Curve/Testing Data

Related Product Information for FNDC5 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- FNDC5antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- FNDC5 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FNDC5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FNDC5 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 47059145
• NCBI GeneID: 384061
• NCBI Accession #: NP_081678.1
• NCBI GenBank Nucleotide #: NM_027402.3
• Molecular Weight: 23,321 Da
• NCBI Official Full Name: fibronectin type III domain-containing protein 5
• NCBI Official Synonym Full Names: fibronectin type III domain containing 5
• NCBI Official Symbol: Fndc5
• NCBI Official Synonym Symbols: PeP; Pxp; C87088; AI836596; 1500001L03Rik
• NCBI Protein Information: fibronectin type III domain-containing protein 5
• UniProt Protein Name: Fibronectin type III domain-containing protein 5
• Protein Family: Fibronectin type III domain-containing protein
• UniProt Gene Name: Fndc5
• UniProt Synonym Gene Names: Frcp2; Pep; PeP
• UniProt Entry Name: FNDC5_MOUSE

Uniprot Description

FNDC5: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: endoplasmic reticulum; extracellular region; integral to membrane; membrane; peroxisome; plasma membrane

Molecular Function: hormone activity; protein binding

Biological Process: response to muscle activity