Testing Data
(Detection of GFAP in rat brain lysate at 4ug/ml.)
Mouse Glial Fibrillary Acidic Protein (GFAP) Monoclonal Antibody | anti-GFAP antibody
Glial Fibrillary Acidic Protein (GFAP) Monoclonal Antibody
Gene Names
GFAP; ALXDRD
Applications
Immunoblot, Western Blot, Immunofluorescence
Synonyms
Glial Fibrillary Acidic Protein (GFAP); Monoclonal Antibody; Glial Fibrillary Acidic Protein (GFAP) Monoclonal Antibody; anti-GFAP antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
This antibody recognizes human, mouse, and rat GFAP.
Form/Format
100ug (1mg/ml) Protein G-purified antibody in PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
Sequence Length
431
Applicable Applications for anti-GFAP antibody
Immunoblot, Immunofluorescence (IF)
Application Notes
Immunoblot: 1-5ug/ml. A band of ~50kDa is detected.
IF: 10ug/ml
Dilute in PBS or medium that is identical to that used in the assay system
IF: 10ug/ml
Dilute in PBS or medium that is identical to that used in the assay system
Immunogen
Synthetic peptide corresponding to aa 411-422 (KTVEMRDGEVIK) of human GFAP. This sequence is 100% identical in mouse and rat
Preparation and Storage
This product is stable for at least one (1) year at-20 degree C.
Testing Data
(Detection of GFAP in rat brain lysate at 4ug/ml.)
Testing Data
(Detection of GFAP in rat brain lysate at 4ug/ml.)
Testing Data
(Detection of GFAP in neuroblastoma cell line SKN-BE at 10ug/ml: DAPI (blue) nuclear stain, Texas Red F actin stain, ATTO 488 (green) GFAP stain.)
Testing Data
(Detection of GFAP in neuroblastoma cell line SKN-BE at 10ug/ml: DAPI (blue) nuclear stain, Texas Red F actin stain, ATTO 488 (green) GFAP stain.)
Related Product Information for anti-GFAP antibody
Glial fibrillary acidic protein (GFAP) is an intermediate filament protein that is expressed by numerous cell types of the central nervous system. It is involved in many important CNS processes, including cell communication and the functioning of the blood brain barrier. It is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell's cytoskeleton. GFAP is thought to help to maintain astrocyte strength and shape. There are multiple disorders associated with improper GFAP regulation. GFAP levels are used as a marker of neurologic damage in adults who suffer strokes and traumatic brain injuries.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,289 Da
NCBI Official Full Name
glial fibrillary acidic protein isoform 2
NCBI Official Synonym Full Names
glial fibrillary acidic protein
NCBI Official Symbol
GFAP
NCBI Official Synonym Symbols
ALXDRD
NCBI Protein Information
glial fibrillary acidic protein
UniProt Protein Name
Glial fibrillary acidic protein
Protein Family
UniProt Gene Name
GFAP
UniProt Synonym Gene Names
GFAP
NCBI Description
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Uniprot Description
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Research Articles on GFAP
Similar Products
Product Notes
The GFAP gfap (Catalog #AAA191359) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glial Fibrillary Acidic Protein (GFAP) can be used in a range of immunoassay formats including, but not limited to, Immunoblot, Immunofluorescence (IF). Immunoblot: 1-5ug/ml. A band of ~50kDa is detected. IF: 10ug/ml Dilute in PBS or medium that is identical to that used in the assay system. Researchers should empirically determine the suitability of the GFAP gfap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glial Fibrillary Acidic Protein (GFAP), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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