Mouse GFAP Monoclonal Antibody | anti-GFAP antibody

GFAP Monoclonal Antibody

Cat. Num. AAA191350

• Gene Names: GFAP; ALXDRD
• Applications: Immunoblot, Western Blot, Immunofluorescence
• Synonyms: GFAP; Monoclonal Antibody; GFAP Monoclonal Antibody; anti-GFAP antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: This antibody recognizes human, mouse and rat GFAP. It cross-reacts with GFAPR416W and other GFAP mutant proteins.
• Form/Format: 100ug (1mg/ml) Protein G-purified antibody in PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
• Sequence Length: 431

• Applicable Applications for anti-GFAP antibody: Immunoblot, Immunofluorescence (IF)
• Application Notes: Immunoblot: 1-4ug/ml. A band of ~50kDa is detected.; IF: 10ug/ml; Dilute in PBS or medium that is identical to that used in the assay system.
• Immunogen: Synthetic peptide corresponding to aa 411-422 (KTVEMRDGEVIK) of human GFAP. This sequence is 100% identical to rat and mouse.
• Preparation and Storage: This product is stable for at least one (1) year at-20 degree C.

Testing Data

(Detection of GFAP in rat brain lysate at 4ug/ml)

Testing Data

(Detection of GFAP in neuroblastoma cell line SKN-BE at 10ug/ml: DAPI (blue) nuclear stain, Texas Red F actin stain, ATTO 488 (green) GFAP stain.)

Related Product Information for anti-GFAP antibody

Glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. Phosphorylation of GFAP, and its association with annexin II-p2 and S-100, regulates GFAP polymerization. One of the first events that occurs during astrocyte proliferation is increased GFAP expression. Antibodies to GFAP have been detected in individuals with dementia, although the significance of this has not been conclusively determined.

NCBI and Uniprot Product Information

• NCBI GI #: 196115290
• NCBI GeneID: 2670
• NCBI Accession #: NP_001124491.1
• NCBI GenBank Nucleotide #: NM_001131019.2
• UniProt Accession #: P14136; Q53H98; Q5D055; Q6ZQS3; Q7Z5J6; Q7Z5J7; Q96KS4; Q96P18; Q9UFD0; B2RD44; D3DX59; E9PAX3
• Molecular Weight: 50,289 Da
• NCBI Official Full Name: glial fibrillary acidic protein isoform 2
• NCBI Official Synonym Full Names: glial fibrillary acidic protein
• NCBI Official Symbol: GFAP
• NCBI Official Synonym Symbols: ALXDRD
• NCBI Protein Information: glial fibrillary acidic protein
• UniProt Protein Name: Glial fibrillary acidic protein
• Protein Family: Glial fibrillary acidic protein
• UniProt Gene Name: GFAP
• UniProt Synonym Gene Names: GFAP

NCBI Description

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.