Immunofluorescence (IF)
(Immunofluorescent detection of Radical fringe homolog expression in HepG2 cells. The anti-Radical fringe homolog primary antibody was used at a concentration of 10 ug/ml.)
Mouse anti-Human Radical fringe homolog Monoclonal Antibody | anti-RFNG antibody
Radical fringe homolog (Beta-1,3-N-acetylglucosaminyltransferase radical fringe, O-fucosylpeptide-3-beta-N-acetylglucosaminyltransferase, RFNG)
Gene Names
RFNG; RFNG
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
Radical fringe homolog; Monoclonal Antibody; Radical fringe homolog (Beta-1; 3-N-acetylglucosaminyltransferase radical fringe; O-fucosylpeptide-3-beta-N-acetylglucosaminyltransferase; RFNG); Anti -Radical fringe homolog (Beta-1; anti-RFNG antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
9G432
Specificity
Recognizes human radical fringe homolog.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Applicable Applications for anti-RFNG antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, ELISA and Western Blot.
Dilution: Immunofluorescence: 10 ug/ml
Dilution: Immunofluorescence: 10 ug/ml
Immunogen
Partial recombinant human radical fringe homolog (aa222-330) with a GST tag.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degree C. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunofluorescence (IF)
(Immunofluorescent detection of Radical fringe homolog expression in HepG2 cells. The anti-Radical fringe homolog primary antibody was used at a concentration of 10 ug/ml.)
Immunofluorescence (IF)
(Immunofluorescent detection of Radical fringe homolog expression in HepG2 cells. The anti-Radical fringe homolog primary antibody was used at a concentration of 10 ug/ml.)
Western Blot (WB)
(Western blot detection Radical fringe homolog expression in HepG2 cells.)
Western Blot (WB)
(Western blot detection Radical fringe homolog expression in HepG2 cells.)
Related Product Information for anti-RFNG antibody
The Drosophila melanogaster 'fringe' gene is a secreted signaling protein that is involved in the formation of boundaries between groups of cells during development. It acts by modulating the activation of te Notch signal transduction pathway. A human radical fringe homolog was identified in 1997. There is evidence to show that radical fringe homolog may be involve in limb formation and in neurogenesis in humans. It is a glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. It is a single-pass type II membrane protein which localises to the golgi apparatus membrane. It belongs to the glycosyltransferase 31 family.
Product Categories/Family for anti-RFNG antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
20,903 Da
NCBI Official Full Name
radical fringe homolog (Drosophila), isoform CRA_d
NCBI Official Synonym Full Names
RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
NCBI Official Symbol
RFNG
NCBI Official Synonym Symbols
RFNG
NCBI Protein Information
beta-1,3-N-acetylglucosaminyltransferase radical fringe; radical fringe homolog; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
UniProt Protein Name
cDNA FLJ36697 fis, clone UTERU2008962, moderately similar to Homo sapiens radical fringe (RFNG) gene
Protein Family
UniProt Entry Name
Q8N9R1_HUMAN
Uniprot Description
RFNG: Glycosyltransferase that initiates the elongation of O- linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. May be involved in limb formation and in neurogenesis. Belongs to the glycosyltransferase 31 family.
Protein type: Membrane protein, integral; Transferase; EC 2.4.1.222; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q25
Cellular Component: extracellular region; integral to Golgi membrane
Molecular Function: metal ion binding; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity
Biological Process: nervous system development; organ morphogenesis; positive regulation of protein binding; metabolic process; cell differentiation; pattern specification process; positive regulation of Notch signaling pathway
Similar Products
Product Notes
The RFNG (Catalog #AAA607259) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Radical fringe homolog (Beta-1,3-N-acetylglucosaminyltransferase radical fringe, O-fucosylpeptide-3-beta-N-acetylglucosaminyltransferase, RFNG) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Radical fringe homolog can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence, ELISA and Western Blot. Dilution: Immunofluorescence: 10 ug/ml. Researchers should empirically determine the suitability of the RFNG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Radical fringe homolog, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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