Rabbit MEK2 Polyclonal Antibody | anti-MEK2 antibody

MEK2 (Phospho-Thr394)

Cat. Num. AAA191020

• Gene Names: MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
• Applications: Western Blot, Immunohistochemistry
• Synonyms: MEK2; Polyclonal Antibody; MEK2 (Phospho-Thr394); anti-MEK2 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous human MEK2 only when phosphorylated at threonine 394.
• Form/Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
• Sequence Length: 400

• Applicable Applications for anti-MEK2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western Blot: use at dilution of 1:500- 1:1,000. A band of ~44kDa is detected.; Immunohistochemistry: use at dilution of 1:50- 1:100.; Dilute in PBS or medium that is identical to that used in the assay system.
• Antigen: Peptide sequence that includes phosphorylation site of threonine (P-G-T(p)-P-T) derived from human MEK2 and conjugated to KLH.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Can be stored at 4 degree C for short-term.

Immunohistochemistry (IHC)

Testing Data

Related Product Information for anti-MEK2 antibody

MEK2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase plays an important role in mitogen growth factor signal transduction. It phosphorylates and activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax.

NCBI and Uniprot Product Information

• NCBI GI #: 13489054
• NCBI GeneID: 5605
• NCBI Accession #: NP_109587.1
• NCBI GenBank Nucleotide #: NM_030662.3
• UniProt Accession #: P36507
• Molecular Weight: 44,424 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 2
• NCBI Official Symbol: MAP2K2
• NCBI Official Synonym Symbols: CFC4; MEK2; MKK2; MAPKK2; PRKMK2
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 2
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 2
• UniProt Gene Name: MAP2K2
• UniProt Synonym Gene Names: MEK2; MKK2; PRKMK2; MAP kinase kinase 2; MAPKK 2; MEK 2

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MEK2: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf and Mos kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK2 and ERK3. A component of MAP kinase signal transduction pathways involved in mitogen growth factor signal transduction.

Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm; cytosol; early endosome; endoplasmic reticulum; focal adhesion; Golgi apparatus; intercellular junction; internal side of plasma membrane; late endosome; microtubule; mitochondrion; nucleus; perinuclear region of cytoplasm; peroxisomal membrane

Molecular Function: MAP kinase kinase activity; PDZ domain binding; protein binding; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; MAPKKK cascade; positive regulation of transcription, DNA-dependent; regulation of stress-activated MAPK cascade

Disease: Cardiofaciocutaneous Syndrome 4