Mouse anti-Human CD2 Monoclonal Antibody | anti-CD2 antibody

Anti-Hu CD2 Alexa Fluor700

• Gene Names: CD2; T11; SRBC; LFA-2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Alexa Fluor700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD2; Monoclonal Antibody; Anti-Hu CD2 Alexa Fluor700; T11; LFA-2; SRBC; CD2 molecule; anti-CD2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: LT2
• Specificity: The antibody LT2 reacts with an extracellular epitope of CD2, a 50kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells; also expressed by all thymocytes.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD2 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: Alexa Fluor700
• Immunogen: Normal human blood lymphocytes.
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD2 (LT2) Alexa Fluor 700 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human CD2 positive lymphocytes (red-filled) from CD2 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD2 (LT2) Alexa Fluor 700 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-CD2 antibody

CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.

References

*Filatov AV, Bachurin PS, Markova NA, Surnakova NE: [A panel of monoclonal antibodies against human lymphocyte antigens] Eksp Onkol. 1989;11(2):28-32., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL:,*Gannon GA, Rhind SG, Suzui M, Zamecnik J, Sabiston BH, Shek PN, Shephard RJ: beta-Endorphin and natural killer cell cytolytic activity during prolonged exercise. Is there a connection Am J Physiol. 1998 Dec;275(6 Pt 2):R1725-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/9843861itool=,*Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106(10):3567-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/16046525itool
*Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21;13(31):4207-13., URL: http://www.ncbi.nlm.nih.gov/sites/entrezDb=pubmed,*Zhu DM, Dustin ML, Cairo CW, Thatte HS, Golan DE: Mechanisms of Cellular Avidity Regulation in CD2-CD58-Mediated T Cell Adhesion. ACS Chem Biol. 2006 Nov 21;1(10):649-58., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Dustin ML: Adhesive bond dynamics in contacts between T lymphocytes and glass-supported planar bilayers reconstituted with the immunoglobulin-related adhesion molecule CD58. J Biol Chem. 1997 Jun 20;272(25):15782-8., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21;13(31):4207-13., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=

NCBI and Uniprot Product Information

• NCBI GI #: 156071472
• NCBI GeneID: 914
• NCBI Accession #: NP_001758.2
• NCBI GenBank Nucleotide #: NM_001767.3
• UniProt Accession #: P06729; Q96TE5
• Molecular Weight: 39,448 Da
• NCBI Official Full Name: T-cell surface antigen CD2
• NCBI Official Synonym Full Names: CD2 molecule
• NCBI Official Symbol: CD2
• NCBI Official Synonym Symbols: T11; SRBC; LFA-2
• NCBI Protein Information: T-cell surface antigen CD2; CD2 antigen (p50), sheep red blood cell receptor; LFA-3 receptor; T-cell surface antigen T11/Leu-5; erythrocyte receptor; lymphocyte-function antigen-2; rosette receptor
• UniProt Protein Name: T-cell surface antigen CD2
• Protein Family: CD2
• UniProt Gene Name: CD2
• UniProt Synonym Gene Names: SRBC
• UniProt Entry Name: CD2_HUMAN

NCBI Description

CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells (summarized by Sewell et al., 1986 [PubMed 3490670]). It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it.[supplied by OMIM, Jul 2010]

Uniprot Description

CD2: CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T- cells, the cytoplasmic domain is implicated in the signaling function.

Protein type: Cell surface; Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: internal side of plasma membrane; cell surface; anchored to plasma membrane; integral to plasma membrane; plasma membrane; extracellular region; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; receptor activity; receptor binding

Biological Process: heterotypic cell-cell adhesion; cell-cell adhesion; cell surface receptor linked signal transduction; T cell activation; apoptosis; natural killer cell activation; regulation of T cell differentiation; blood coagulation; lipid raft polarization; positive regulation of tumor necrosis factor production; leukocyte migration; positive regulation of myeloid dendritic cell activation