Rabbit GRO gamma Polyclonal Antibody | anti-CXCL3 antibody

Anti-GRO gamma Antibody

Cat. Num. AAA178441

• Gene Names: Cxcl3; Dcip1; Gm1960
• Reactivity: Mouse; No cross reactivity with other proteins.
• Applications: Western Blot, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: GRO gamma; Polyclonal Antibody; Anti-GRO gamma Antibody; Cinc 2; Cinc2; Cinc-2; CINC 2b; CINC2b; CINC-2b; CXCL 3; CXCL3; Dcip1; Gm1960; GRO protein gamma; GRO-gamma; GRO-gamma(1-73); GRO-gamma(5-73); GRO3; GRO3 oncogene; GROG; MGSA gamma; MIP 2b; MIP2b; MIP-2b; MIP2-beta; MIP 2B; MIP2B; MIP-2B; SCYB3; Q6W5C0; C-X-C motif chemokine 3; C-X-C motif chemokine ligand 3; anti-CXCL3 antibody

• Host: Rabbit
• Reactivity: Mouse; No cross reactivity with other proteins.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 100

• Applicable Applications for anti-CXCL3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Western Blot: 0.1-0.5mug/ml; Tested Species: Mouse; ELISA: 0.1-0.5mug/ml; Tested Species: Mouse; Tested Species:In-house tested species with positive results.; Other applications have not been tested.
• Immunogen: E Coli-derived mouse GRO gamma recombinant protein (Position: S32-S100). Mouse GRO gamma shares 60.6% and 90.6% amino acid (aa) sequence identity with human and rat GRO gamma, respectively.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of GRO gamma expression in mouse lung extract (lane 1) and mouse spleen extract (lane 2). GRO gamma at 25KD was detected using rabbit anti- GRO gamma Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Related Product Information for anti-CXCL3 antibody

Rabbit IgG polyclonal antibody for C-X-C motif chemokine 3 (CXCL3) detection.
Background: Chemokine (C-X-C motif) ligand 3 (CXCL3), also known as GRO protein gamma (GROg) and macrophage inflammatory protein-2-beta (MIP2b), is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.

References

1. Ahuja SK, Murphy PM (August 1996). "The CXC chemokines growth-regulated oncogene (GRO) alpha, GRObeta, GROgamma, neutrophil-activating peptide-2, and epithelial cell-derived neutrophil-activating peptide-78 are potent agonists for the type B, but not the type A, human interleukin-8 receptor". J. Biol. Chem. 271 (34): 20545-50.
2. Farioli-Vecchioli S, Cinà I, Ceccarelli M, Micheli L, Leonardi L, Ciotti MT, De Bardi M, Di Rocco C, Pallini R, Cavallaro S, Tirone F (October 2012). "Tis21 knock-out enhances the frequency of medulloblastoma in patched1 heterozygous mice by inhibiting the cxcl3-dependent migration of cerebellar neurons". The Journal of neuroscience : the official journal of the Society for Neuroscience 32 (44): 15547-15564.
3. Smith DF, Galkina E, Ley K, Huo Y (November 2005). "GRO family chemokines are specialized for monocyte arrest from flow". Am. J. Physiol. Heart Circ. Physiol. 289 (5): H1976-84.

NCBI and Uniprot Product Information

• NCBI GI #: 42600573
• NCBI GeneID: 330122
• NCBI Accession #: NP_976065.1
• NCBI GenBank Nucleotide #: NM_203320.3
• UniProt Accession #: Q6W5C0; Q3UNK9
• Molecular Weight: 10,686 Da
• NCBI Official Full Name: C-X-C motif chemokine 3
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 3
• NCBI Official Symbol: Cxcl3
• NCBI Official Synonym Symbols: Dcip1; Gm1960
• NCBI Protein Information: C-X-C motif chemokine 3
• UniProt Protein Name: C-X-C motif chemokine 3
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: Cxcl3
• UniProt Entry Name: CXCL3_MOUSE

NCBI Description

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. [provided by RefSeq, May 2013]

Uniprot Description

CXCL1: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular space

Molecular Function: chemokine activity; CXCR chemokine receptor binding; protein binding

Biological Process: elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; neutrophil chemotaxis; regulation of cell proliferation; response to lipopolysaccharide