Rabbit Sodium/hydrogen exchanger 1 Polyclonal Antibody | anti-SLC9A1 antibody

Anti-SLC9A1 Antibody

Cat. Num. AAA177483

• Gene Names: SLC9A1; APNH; NHE1; NHE-1; PPP1R143
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Sodium/hydrogen exchanger 1; Polyclonal Antibody; Anti-SLC9A1 Antibody; amiloride-sensitive antibody; APNH antibody; APNH1 antibody; FLJ42224 antibody; Na Li countertransporter antibody; Na(+)/H(+) antiporter antibody; Na(+)/H(+) exchanger 1 antibody; Na+ H+ antiporter amiloride-sensitive antibody; Na+ H+ antiporter antibody; Na+ H+ exchanger 1 antibody; NHE-1 antibody; NHE1 antibody; OTTHUMP00000004468 antibody; SL9A1_HUMAN antibody; SLC9A1 antibody; Sodium hydrogen exchanger 1 antibody; Sodium/hydrogen exchanger 1 antibody; solute carrier family 9 antibody; Solute carrier family 9 member 1 antibody; Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive antibody; Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1 antibody; Solute carrier family 9 subfamily A member 1 antibody; solute carrier family 9; subfamily A (NHE1; cation proton antiporter 1); member 1; anti-SLC9A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with othee protiens
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 815

• Applicable Applications for anti-SLC9A1 antibody: Western Blot (WB), Immunohistochemistry (IHC-P)
• Application Notes: Western Blot: ; Concentration: 0.1-0.5ug/ml; Tested Species: Human, Rat; ; Immunohistochemistry (Paraffin-embedded Section): ; Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat; ; WB: The detection limit for SLC9A1 is approximately 0.25ng/lane under reducing conditions.; Tested Species: In house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer , pH 6.0 for 20mins is required for the staining of formalin/paraffin sections.; ; Other applications have not been tested; Optimal dilutions should be determined by the end users.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: E.coli-derived human SLC9A1 recombinant protein (Position: H543-Q815). Human SLC9A1 shares 92% and 93% amino acid (aa) sequences identity with mouse and rat SLC9A1, respectively.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Testing Data

(Anti-SLC9A1 Picoband antibody, MBS177483-1.jpgAll lanes: Anti SLC9A1 (MBS177483) at 0.5ug/mlWB: Recombinant Human Sodium Protein 0.5ngPredicted bind size: 37KDObserved bind size: 37KD )

Testing Data

(Anti-SLC9A1 Picoband antibody, MBS177483-2.jpgAll lanes: Anti SLC9A1 (MBS177483) at 0.5ug/mlLane 1: PC-12 Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 91KDObserved bind size: 91KD )

Immunohistochemistry (IHC)

(Anti-SLC9A1 Picoband antibody, MBS177483-3.JPGIHC(P): Rat Kidney Tissue )

Immunohistochemistry (IHC)

(Anti-SLC9A1 Picoband antibody, MBS177483-4.JPGIHC(P): Human Mammary Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-SLC9A1 Picoband antibody, MBS177483-5.JPGIHC(P): Mouse Kidney Tissue )

Related Product Information for anti-SLC9A1 antibody

Background: NHE1, also known as SLC9A1, is an isoform of sodium–hydrogen antiporter that in humans is encoded by the SLC9A1 gene. It is mapped to 1p36.11. NHE1 is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others. The plasma membrane ion exchanger NHE1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. And a structural role for NHE1 in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. NHE1 is a plausible candidate gene for human essential hypertension.

NCBI and Uniprot Product Information

• NCBI GI #: 27777632
• NCBI GeneID: 6548
• NCBI Accession #: NP_003038.2
• NCBI GenBank Nucleotide #: NM_003047.4
• UniProt Accession #: P19634; Q96EM2; B1ALD6; D3DPL4
• NCBI Official Full Name: sodium/hydrogen exchanger 1
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1
• NCBI Official Symbol: SLC9A1
• NCBI Official Synonym Symbols: APNH; NHE1; NHE-1; PPP1R143
• NCBI Protein Information: sodium/hydrogen exchanger 1; Na(+)/H(+) exchanger 1; Na-Li countertransporter; protein phosphatase 1, regulatory subunit 143; solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive); solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+, amiloride sensitive)
• UniProt Protein Name: Sodium/hydrogen exchanger 1
• Protein Family: Sodium/hydrogen exchanger
• UniProt Gene Name: SLC9A1
• UniProt Synonym Gene Names: APNH1; NHE1; NHE-1
• UniProt Entry Name: SL9A1_HUMAN

NCBI Description

This gene encodes a Na+/H+ antiporter that is a member of the solute carrier family 9. The encoded protein is a plasma membrane transporter that is expressed in the kidney and intestine. This protein plays a central role in regulating pH homeostasis, cell migration and cell volume. This protein may also be involved in tumor growth. [provided by RefSeq, Sep 2011]

Uniprot Description

NHE1: sodium/hydrogen exchanger 1. Belongs to the Na(+)/H(+) exchanger family. Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Transporter; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: endoplasmic reticulum membrane; cell surface; focal adhesion; mitochondrion; integral to plasma membrane; endoplasmic reticulum; basolateral plasma membrane; T-tubule; integral to membrane; lipid raft; nucleoplasm; lamellipodium; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; plasma membrane

Molecular Function: protein binding, bridging; calmodulin binding; protein binding; phosphatidylinositol-4,5-bisphosphate binding; protein complex scaffold; solute:hydrogen antiporter activity; calcium-dependent protein binding; sodium:hydrogen antiporter activity; protein phosphatase 2B binding

Biological Process: glycosaminoglycan metabolic process; positive regulation of NFAT protein import into nucleus; pathogenesis; cardiac muscle cell differentiation; hyaluronan catabolic process; regulation of pH; positive regulation of action potential; cell growth; transmembrane transport; response to drug; cellular sodium ion homeostasis; cell migration; maintenance of cell polarity; positive regulation of cell growth; protein oligomerization; cellular response to insulin stimulus; regulation of stress fiber formation; regulation of intracellular pH; carbohydrate metabolic process; regulation of focal adhesion formation; ion transport; positive regulation of transcription from RNA polymerase II promoter; regulation of sensory perception of pain; response to acidity; hyaluronan metabolic process; negative regulation of apoptosis

Disease: Lichtenstein-knorr Syndrome