Typical Testing Data/Standard Curve (for reference only)
Human Sodium/Hydrogen Exchanger 1 (NHE1) RTU ELISA Kit | NHE1 rtu elisa kit
Human Sodium/Hydrogen Exchanger 1 (NHE1) ELISA Kit
Gene Names
SLC9A1; APNH; NHE1; LIKNS; NHE-1; PPP1R143
Reactivity
Human
Synonyms
Sodium/Hydrogen Exchanger 1 (NHE1); Human Sodium/Hydrogen Exchanger 1 (NHE1) ELISA Kit; SLC9A1; APNH; Solute Carrier Family 9 Member 1; Sodium/Hydrogen Exchanger; Antiporter; Na+/H+; Amiloride Sensitive; Na(+)/H(+) antiporter; amiloride-sensitive; NHE1 rtu elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of NHE1. No significant cross-reactivity or interference between NHE1 and analogues was observed.
Sequence Length
815
Samples
Human tissue homogenates, cell lysates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
< 0.064ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NHE1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NHE1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NHE1 rtu elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NHE1 in human tissue homogenates, cell lysates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NHE1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NHE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NHE1, biotin-conjugated antibody and enzyme- conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NHE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NHE1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NHE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NHE1, biotin-conjugated antibody and enzyme- conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NHE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,013 Da
NCBI Official Full Name
sodium/hydrogen exchanger 1
NCBI Official Synonym Full Names
solute carrier family 9 member A1
NCBI Official Symbol
SLC9A1
NCBI Official Synonym Symbols
APNH; NHE1; LIKNS; NHE-1; PPP1R143
NCBI Protein Information
sodium/hydrogen exchanger 1
UniProt Protein Name
Sodium/hydrogen exchanger 1
UniProt Gene Name
SLC9A1
UniProt Synonym Gene Names
APNH1; NHE1; NHE-1
NCBI Description
This gene encodes a Na+/H+ antiporter that is a member of the solute carrier family 9. The encoded protein is a plasma membrane transporter that is expressed in the kidney and intestine. This protein plays a central role in regulating pH homeostasis, cell migration and cell volume. This protein may also be involved in tumor growth. [provided by RefSeq, Sep 2011]
Uniprot Description
Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
Research Articles on NHE1
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Product Notes
The Human NHE1 slc9a1 (Catalog #AAA4501373) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA4501373 RTU ELISA Kit recognizes Human NHE1. It is sometimes possible for the material contained within the vial of "Sodium/Hydrogen Exchanger 1 (NHE1), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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