Human Sodium/Hydrogen Exchanger 1 (NHE1) ELISA Kit | NHE1 elisa kit

Human Sodium/Hydrogen Exchanger 1 (NHE1) ELISA Kit

Cat. Num. AAA450790

• Gene Names: SLC9A1; APNH; NHE1; LIKNS; NHE-1; PPP1R143
• Reactivity: Human
• Synonyms: Sodium/Hydrogen Exchanger 1 (NHE1); Human Sodium/Hydrogen Exchanger 1 (NHE1) ELISA Kit; SLC9A1; APNH; Solute Carrier Family 9 Member 1; Sodium/Hydrogen Exchanger; Antiporter; Na+/H+; Amiloride Sensitive; Na(+)/H(+) antiporter; amiloride-sensitive; NHE1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NHE1. No significant cross-reactivity or interference between NHE1 and analogues was observed.
• Sequence Length: 815

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.064ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NHE1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NHE1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NHE1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NHE1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NHE1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NHE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NHE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NHE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27777632
• NCBI GeneID: 6548
• NCBI Accession #: NP_003038.2
• NCBI GenBank Nucleotide #: NM_003047.4
• UniProt Accession #: P19634; Q96EM2; B1ALD6; D3DPL4
• Molecular Weight: 61,013 Da
• NCBI Official Full Name: sodium/hydrogen exchanger 1
• NCBI Official Synonym Full Names: solute carrier family 9 member A1
• NCBI Official Symbol: SLC9A1
• NCBI Official Synonym Symbols: APNH; NHE1; LIKNS; NHE-1; PPP1R143
• NCBI Protein Information: sodium/hydrogen exchanger 1
• UniProt Protein Name: Sodium/hydrogen exchanger 1
• UniProt Gene Name: SLC9A1
• UniProt Synonym Gene Names: APNH1; NHE1; NHE-1

NCBI Description

This gene encodes a Na+/H+ antiporter that is a member of the solute carrier family 9. The encoded protein is a plasma membrane transporter that is expressed in the kidney and intestine. This protein plays a central role in regulating pH homeostasis, cell migration and cell volume. This protein may also be involved in tumor growth. [provided by RefSeq, Sep 2011]

Uniprot Description

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.