Mouse TREM-1 ELISA Kit | TREM1 elisa kit

Mouse TREM-1 PicoKine ELISA Kit

Cat. Num. AAA177129

• Reactivity: Mouse
• Synonyms: TREM-1; Mouse TREM-1 PicoKine ELISA Kit; Triggering receptor expressed on myeloid cells 1; triggering receptor expressed on myeloid cells 1; CD354; TREM 1Triggering receptor expressed on monocytes 1; Triggering receptor TREM 1; Triggering receptor TREM1; TREM1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse TREM-1
• Sequence Length: 230

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA), Cell lysates and tissue homogenates
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse TREM-1 PicoKine ELISA Kit standard curve )

Related Product Information for TREM1 elisa kit

Principle of the assay: mouse TREM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TREM-1 has been precoated onto 96-well plates. Standards(NSO, A21-S202) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TREM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TREM-1 amount of sample captured in plate.
Background: Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide(LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8(IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1(MCP1, or SCYA2) and tumor necrosis factor(TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules(e.g., ITGB1) and costimulatory molecules(e.g., CD40). Trem1 is associated with DAP12(TYROBP), a molecule frequently associated with activating receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 81175033
• NCBI GeneID: 58217
• UniProt Accession #: Q9JKE2; Q3TAL7
• Molecular Weight: 25,409 Da
• NCBI Official Full Name: Triggering receptor expressed on myeloid cells 1
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 1
• NCBI Official Symbol: Trem1
• NCBI Protein Information: triggering receptor expressed on myeloid cells 1; triggering receptor expressed in monocytes 1
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 1
• Protein Family: Triggering receptor expressed on myeloid cells
• UniProt Gene Name: Trem1
• UniProt Synonym Gene Names: TREM-1
• UniProt Entry Name: TREM1_MOUSE

Uniprot Description

TREM1: Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: membrane; cell; integral to membrane

Biological Process: neutrophil chemotaxis; cell migration; cytokine metabolic process; cytokine secretion during immune response; chemokine metabolic process