Testing Data
(Staining of J774 cells with Rat anti Mouse TREM-1:Alexa Fluor ?647 (MBS219711A647))
Rat TREM-1 Monoclonal Antibody | anti-TREM-1 antibody
RAT ANTI MOUSE TREM-1
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Synonyms
TREM-1; Monoclonal Antibody; RAT ANTI MOUSE TREM-1; anti-TREM-1 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
L5-B8.2A12.3A12
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
230
Applicable Applications for anti-TREM-1 antibody
Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/100
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/100
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Immunogen
Mouse TREM-1 Fc fusion protein
Buffer Solution
Buffer Solution
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Staining of J774 cells with Rat anti Mouse TREM-1:Alexa Fluor ?647 (MBS219711A647))
Testing Data
(Staining of J774 cells with Rat anti Mouse TREM-1:Alexa Fluor ?647 (MBS219711A647))
Testing Data
(Staining of J774 cells with Rat anti Mouse TREM-1:Alexa Fluor 488 (MBS219711A488))
Testing Data
(Staining of J774 cells with Rat anti Mouse TREM-1:Alexa Fluor 488 (MBS219711A488))
Testing Data
(Staining of J774 cells with Rat anti Mouse TREM-1:RPE (MBS224418))
Testing Data
(Staining of J774 cells with Rat anti Mouse TREM-1:RPE (MBS224418))
Related Product Information for anti-TREM-1 antibody
Rat anti Mouse Trem-1 antibody, clone L5-B8.2A12.3A12 recognizes mouse triggering receptor expressed on myeloid cells 1 (TREM-1), a 30kD member of the immunoglobulin superfamily expressed on neutrophils and a subset of monocytes. TREM-1 is involved in the microbial inflammatory response. It acts in conjunction with the adaptor protein DAP12 to activate neutrophils and monocytes, leading to the secretion of pro-inflammatory mediators and enhancement of the inflammatory response. Microbial infection also induces the release of soluble form of TREM-1 from monocytes, which is reported to act as an inhibitor of TREM-1. TREM-1 mediates the septic shock pathway, and blocking TREM-1 has been reported to reduce inflammation and increase survival in certain models of bacterial infection.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,409 Da
NCBI Official Full Name
triggering receptor expressed on myeloid cells 1
NCBI Official Synonym Full Names
triggering receptor expressed on myeloid cells 1
NCBI Official Symbol
Trem1
NCBI Protein Information
triggering receptor expressed on myeloid cells 1; triggering receptor expressed in monocytes 1
UniProt Protein Name
Triggering receptor expressed on myeloid cells 1
UniProt Gene Name
Trem1
UniProt Synonym Gene Names
TREM-1
UniProt Entry Name
TREM1_MOUSE
Uniprot Description
TREM1: Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.
Cellular Component: membrane; cell; integral to membrane
Biological Process: neutrophil chemotaxis; cell migration; cytokine metabolic process; cytokine secretion during immune response; chemokine metabolic process
Research Articles on TREM-1
Similar Products
Product Notes
The TREM-1 trem1 (Catalog #AAA219355) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TREM-1 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections. Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/100. Researchers should empirically determine the suitability of the TREM-1 trem1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TREM-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
