TREM-1 elisa kit

Human TREM-1 PicoKine ELISA Kit

Cat. Num. AAA1751543

• Gene Names: TREM1; CD354; TREM-1
• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Synonyms: TREM-1; Human TREM-1 PicoKine ELISA Kit; Human TREM-1; Triggering receptor expressed on myeloid cells 1; Triggering receptor expressed on monocytes 1; CD354; TREM1; TREM-1 elisa kit

• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Human TNFRSF25
• Sequence Length: 150

• Samples: Cell Culture Supernates, Cell Lysates, Serum And Plasma (Heparin, Edta)
• Detection Range: 312pg/ml-20000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months.; Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TREM-1 elisa kit

Principle of the Assay: The Picokine Human TNFRSF25 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TNFRSF25 with a 96-well strip plate that is pre-coated with antibody specific for TNFRSF25. The detection antibody is a biotinylated antibody specific for TNFRSF25. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TNFRSF25 with immunogen: Expression system for standard: NSO, Immunogen sequence: Q25-F201. The kit is analytically validated with ready to use reagents. To measure Human TNFRSF25, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human TNFRSF25 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TNFRSF25 in the sample!!Background/Introduction: Death receptor 3 (DR3), also known as tumor necrosis factor receptor superfamily member 25 (TNFRSF25), is a cell surface receptor of the tumor necrosis factor receptor superfamily which mediates apoptotic signalling and differentiation. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.

NCBI and Uniprot Product Information

• NCBI GI #: 336088680
• NCBI GeneID: 54210
• NCBI Accession #: NP_001229518.1
• NCBI GenBank Nucleotide #: NM_001242589.2
• UniProt Accession #: Q9NP99; Q53FL8; Q5T2C9; Q86YU1; B4DWG2; K7EJW1
• Molecular Weight: 17,563 Da
• NCBI Official Full Name: triggering receptor expressed on myeloid cells 1 isoform 2
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 1
• NCBI Official Symbol: TREM1
• NCBI Official Synonym Symbols: CD354; TREM-1
• NCBI Protein Information: triggering receptor expressed on myeloid cells 1
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 1
• UniProt Gene Name: TREM1
• UniProt Synonym Gene Names: TREM-1

NCBI Description

This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock.