Testing Data
Human ANGPTL3 ELISA Kit | ANGPTL3 elisa kit
Human ANGPTL3 Quick ELISA Kit
O.D.|0.000|0.240|0.454|0.818|1.432|2.188|2.521|2.873
n|16|16|16|
Mean(pg/ml)|712|1841|9677|
Standard deviation|31.33|106.78|522.56|
CV(%)|4.4%|5.8%|5.4%|
n|24|24|24
Mean(pg/ml)|751|1716|9563
Standard deviation|44.31|125.27|592.91
CV(%)|5.9%|7.3%|6.2%
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 712|718|645|684|690|30.35|4.4%
Sample 2: 1841|2134|2209|1998|2046|130.91|6.4%
Sample 3: 9677|8512|10354|8424|9242|822.52|8.9%
*number of samples for each test n=16.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
To measure Human ANGPTL3, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human ANGPTL3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human ANGPTL3 in the sample.
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metablism, while the C-terminal chain may be involved in angiogenesis. Mutations in this gene cause familial hypobetalipoproteinemia type 2. [provided by RefSeq, Feb 2013]
Uniprot Description
ANGPTL3: Defects in ANGPTL3 are the cause of familial hypobetalipoproteinemia type 2 (FHBL2); also called combined hypobetalipoproteinemia familial. FHBL2 is a disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Affected individuals present with combined hypolipidemia, consisting of extremely low plasma levels of LDL cholesterol, HDL cholesterol, and triglycerides.
Protein type: Secreted; Cell adhesion; Inhibitor; Motility/polarity/chemotaxis; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1p31.3
Cellular Component: extracellular space; cell surface
Molecular Function: integrin binding; enzyme inhibitor activity; phospholipase inhibitor activity; growth factor activity
Biological Process: integrin-mediated signaling pathway; cholesterol metabolic process; phospholipid catabolic process; glycerol metabolic process; cell-matrix adhesion; lipid homeostasis; positive regulation of lipid catabolic process; fatty acid metabolic process; signal transduction; sequestering of lipid; positive regulation of angiogenesis; cholesterol homeostasis; acylglycerol homeostasis; phospholipid metabolic process; artery morphogenesis; negative regulation of lipoprotein lipase activity; positive regulation of cell migration; phospholipid homeostasis
Disease: Hypobetalipoproteinemia, Familial, 2
Research Articles on ANGPTL3
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Product Notes
The Human ANGPTL3 angptl3 (Catalog #AAA1753951) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1753951 ELISA Kit recognizes Human ANGPTL3. It is sometimes possible for the material contained within the vial of "ANGPTL3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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