Western Blot (WB)
(Western Blot analysis of Coilin on rat kidney tissue lysates using anti-Coilin at 1/500 dilution.)
Rabbit anti-Human Coilin Polyclonal Antibody | anti-Coilin antibody
Coilin Polyclonal Antibody
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Coilin; Polyclonal Antibody; Coilin Polyclonal Antibody; COIL; CLN80; CLN 80; Coilin p80; p80; P80 coilin; anti-Coilin antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Coilin polyclonal antibody detects endogenous levels of Coilin protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
576
Applicable Applications for anti-Coilin antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2,000
FC/FACS: 1:50-1:100
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of Coilin on rat kidney tissue lysates using anti-Coilin at 1/500 dilution.)
Western Blot (WB)
(Western Blot analysis of Coilin on rat kidney tissue lysates using anti-Coilin at 1/500 dilution.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Jurkat cells with Coilin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Jurkat cells with Coilin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-Coilin antibody
Coilin protein is one of the main molecular components of Cajal bodies (CBs). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. In addition to its structural role, coilin acts as glue to connect the CB to the nucleolus. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome and chromosome.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
63kDa (Predicted Band Size)
NCBI Official Full Name
coilin
UniProt Protein Name
Coilin
Protein Family
UniProt Gene Name
COIL
UniProt Synonym Gene Names
CLN80
UniProt Entry Name
COIL_HUMAN
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Product Notes
The Coilin coil (Catalog #AAA3012493) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Coilin Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Coilin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). WB: 1:500-1:2,000 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the Coilin coil for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Coilin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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