Rabbit anti-Mouse, Rat PBP Polyclonal Antibody | anti-PEBP1 antibody

Anti-PBP antibody

Cat. Num. AAA175385

• Gene Names: Pebp1; Pbp; HCNP; Pbp1; Pbpr; Rkip
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: PBP; Polyclonal Antibody; Anti-PBP antibody; Phosphatidylethanolamine-binding protein 1(PEBP-1); phosphatidylethanolamine binding protein 1; HCNP antibody; HCNPpp antibody; Hippocampal cholinergic neurostimulating peptide antibody; Neuropolypeptide h3 antibody; PEBP antibody; PEBP 1 antibody; PEBP-1 antibody; PEBP1 antibody; PEBP1_HUMAN antibody; Phosphatidylethanolamine binding protein antibody; Phosphatidylethanolamine binding protein 1 antibody; Prostatic binding protein antibody; Prostatic-binding protein antibody; R kip antibody; Raf kinase inhibitor protein antibody; Raf kinase inhibitory protein antibody; RKIP antibody; anti-PEBP1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 187

• Applicable Applications for anti-PEBP1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse PBP (15-29aa QEVDEPPQHALRVDY), identical to the related rat sequence.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-PBP antibody, MBS175385, Western blottingAll lanes: Anti PBP (MBS175385) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: Rat Liver Tissue Lysate at 50ugPredicted bind size: 21KDObserved bind size: 21KD)

Immunohistochemistry (IHC)

(Anti-PBP antibody, MBS175385, IHC(P)IHC(P): Rat Kidney Tissue )

Related Product Information for anti-PEBP1 antibody

Description: Rabbit IgG polyclonal antibody for Phosphatidylethanolamine-binding protein 1(PEBP1) detection. Tested with WB, IHC-P in Mouse, Rat.
Background: PEBP1(Phosphatidylethanolamine-binding protein 1), also called PBP, RKIP, inhibits the phosphorylation and activation of MEK by RAF1. PEBP1 is identical to the phosphatidylethanolamine-binding protein(PBP) with a relative molecular mass of 23 kD. The PEBP1 gene is mapped on 12q24.23. PEBP1 coimmunoprecipitates with RAF1 and MEK from cell lysates and colocalizes with RAF1 when examined by confocal microscopy. PEBP1 overexpression interferes with the activation of MEK and ERK, induction of AP1-dependent reporter genes, and transformation elicited by an oncogenically activated RAF1 kinase. PEBP1 expression was rapidly upregulated during induction of chemotherapy-triggered apoptosis in human prostate and breast cancer cell lines, and maximal RKIP expression correlated perfectly with the onset of apoptosis by Chatterjee et al(2004). RKIP depletion decreased the mitotic index, the number of metaphase cells, traversal times from nuclear envelope breakdown to anaphase, and an override of mitotic checkpoints induced by spindle poisons.

NCBI and Uniprot Product Information

• NCBI GI #: 29840839
• NCBI GeneID: 23980
• UniProt Accession #: P70296; Q9D8G9; Q9JJ58
• Molecular Weight: 20,830 Da
• NCBI Official Full Name: Phosphatidylethanolamine-binding protein 1
• NCBI Official Synonym Full Names: phosphatidylethanolamine binding protein 1
• NCBI Official Symbol: Pebp1
• NCBI Official Synonym Symbols: Pbp; HCNP; Pbp1; Pbpr; Rkip
• NCBI Protein Information: phosphatidylethanolamine-binding protein 1; HCNPpp; PEBP-1; Raf-1 inhibitor protein; Raf-1 kinase inhibitor protein
• UniProt Protein Name: Phosphatidylethanolamine-binding protein 1
• Protein Family: Phosphatidylethanolamine-binding protein
• UniProt Gene Name: Pebp1
• UniProt Synonym Gene Names: Pbp; Pebp; PEBP-1; HCNP
• UniProt Entry Name: PEBP1_MOUSE

Uniprot Description

RKIP: a metastasis suppressor that regulates extracellular matrix remodeling and tumor survival. Binds to and inhibits Raf-1. Phosphorylation by classic and atypical but not novel PKC isoforms dissociates this complex, relieving inhibition of the Raf/MAP kinase signaling cascade. Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. Cleaved into hippocampal cholinergic neurostimulating peptide (HCMP) that appears to be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Expressed in brain. Increased expression in aged senescence-accelerated mice.

Protein type: Protein kinase, regulatory subunit; Lipid-binding

Cellular Component: Golgi apparatus; extracellular space; neuron projection; cell surface; rough endoplasmic reticulum; mitochondrion; mitochondrial outer membrane; synaptic vesicle; cell soma; apical part of cell; cytoplasm; nerve terminal; nucleus; myelin sheath

Molecular Function: serine-type endopeptidase inhibitor activity; enzyme binding; protease inhibitor activity; nucleotide binding; mitogen-activated protein kinase binding; lipid binding; kinase binding; protein kinase binding; ATP binding; receptor binding

Biological Process: positive regulation of mitosis; negative regulation of protein amino acid phosphorylation; negative regulation of MAPKKK cascade; regulation of neurotransmitter levels; MAPKKK cascade; negative regulation of peptidase activity; regulation of the force of heart contraction; sperm capacitation