Rabbit anti-Human USP21 Polyclonal Antibody | anti-USP21 antibody

USP21, ID (Ubiquitin Carboxyl-terminal Hydrolase 21, Ubiquitin Thioesterase 21, Ubiquitin-specific-Processing Protease 21, Deubiquitinating Enzyme 21, PP1490, USP23) (Azide free) (HRP)

Cat. Num. AAA6505248

• Gene Names: USP21; USP16; USP23
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: USP21; Polyclonal Antibody; ID (Ubiquitin Carboxyl-terminal Hydrolase 21; Ubiquitin Thioesterase 21; Ubiquitin-specific-Processing Protease 21; Deubiquitinating Enzyme 21; PP1490; USP23) (Azide free) (HRP); anti-USP21 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human USP21.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 565

• Applicable Applications for anti-USP21 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the central region of human USP21 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hUSP21-L241 in NCI-H460 cell line lysates (35ug/lane). USP21 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-USP21 antibody

Antibodies; Proteins

References

1. Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003). 2. Gong, L., et al., J. Biol. Chem. 275(19):14212-14216 (2000). 3. Hillier, L.D., et al., Genome Res. 6(9):807-828 (1996). 4. Smith, T.S., et al., Biochim. Biophys. Acta 1490(1-2), 184-188 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 74027267
• NCBI GeneID: 27005
• NCBI Accession #: NP_036607
• NCBI GenBank Nucleotide #: NP_036607.3
• UniProt Accession #: Q9UK80
• Molecular Weight: 62.656
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 21 isoform c
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 21
• NCBI Official Symbol: USP21
• NCBI Official Synonym Symbols: USP16; USP23
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 21
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 21
• UniProt Gene Name: USP21
• UniProt Synonym Gene Names: USP23
• UniProt Entry Name: UBP21_HUMAN

NCBI Description

This gene encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016]

Uniprot Description

USP21: Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination. Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates. Belongs to the peptidase C19 family. USP21 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Protease; EC 3.4.19.12; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: cytoplasm; nucleoplasm; plasma membrane

Molecular Function: cysteine-type peptidase activity; metal ion binding; NEDD8-specific protease activity; protein binding; transcription coactivator activity; ubiquitin-specific protease activity

Biological Process: histone deubiquitination; neurite development; positive regulation of transcription, DNA-dependent; transcription, DNA-dependent; ubiquitin-dependent protein catabolic process