PEBP1 Human Recombinant Protein | PEBP1 recombinant protein

PEBP1, Recombinant Human (Prostatic Binding Protein, PBP, Hippocampal Cholinergic Neurostimulating Peptide, HCNP, HCNPpp, Neuropolypeptide h3, Phosphatidylethanolamine Binding Protein, PEBP, Phosphatidylethanolamine Binding Protein 1, PEBP1, PEBP-1, Raf K

Cat. Num. AAA635945

• Gene Names: PEBP1; PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1
• Applications: Specific methodologies for the use of this product have not been tested.
• Purity: Affinity Purified; ~95% by SDS-PAGE Purified by immunoaffinity chromatography.
• Synonyms: PEBP1 Human; PEBP1; Recombinant Human (Prostatic Binding Protein; PBP; Hippocampal Cholinergic Neurostimulating Peptide; HCNP; HCNPpp; Neuropolypeptide h3; Phosphatidylethanolamine Binding Protein; PEBP; Phosphatidylethanolamine Binding Protein 1; PEBP-1; Raf K; PEBP1 recombinant protein

• Host: Recombinant human PEBP1, 1aa-187, expressed in E. coli
• Purity/Purification: Affinity Purified; ~95% by SDS-PAGE Purified by immunoaffinity chromatography.
• Form/Format: Supplied as iquid in 20mM Tris-HCl buffer, pH8.0, 1mM DTT, 10% glycerol
• Sequence Positions: 1-187
• Sequence: MPVDLSKWSG PLSLQEVDEQ PQHPLHVTYA GAAVDELGKV LTPTQVKNRP TSISWDGLDS GKLYTLVLTD PDAPSRKDPK YREWHHFLVV NMKGNDISSG TVLSDYVGSG PPKGTGLHRY VWLVYEQDRP LKCDEPILSN RSGDHRGKFK VASFRKKYEL RAPVAGTCYQ AEWDDYVPKL; YEQLSGK
• Sequence Length: 187

• Applicable Applications for PEBP1 recombinant protein: Specific methodologies for the use of this product have not been tested.
• Application Notes: Optimal dilutions to be determined by the researcher.
• Source Note: Recombinant human PEBP1, 1aa-187, expressed in E. coli
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(15% SDS-PAGE (3ug))

Related Product Information for PEBP1 recombinant protein

Phosphatidylethanolamine binding protein 1 (PEBP1), also known as Raf kinase inhibitor protein (RKIP), is a member of the phosphatidylethanolamine-binding protein family and a serine protease inhibitor which inhibits thrombin, neuropsin. PEBP1 plays a pivotal modulatory role in several protein kinase signaling cascades. Protein kinase C (PKC) phosphorylates PEBP1, resulting in release of Raf-1 and activation of MEK and ERK. Expressed in many tissues, PEBP1 is implicated in the regulation of such physiological processes as membrane biosynthesis, spermatogenesis, neural development, and metastasis suppression. Recombinant human PEBP1, expressed in E. coli and purified by using conventional chromatography techniques.

Product Categories/Family for PEBP1 recombinant protein

Molecular Biology; MB-Protein Kinase Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 4505621
• NCBI GeneID: 5037
• NCBI Accession #: NP_002558.1
• NCBI GenBank Nucleotide #: NM_002567.2
• UniProt Accession #: P30086; B2R4S1
• Molecular Weight: 21kD
• NCBI Official Full Name: phosphatidylethanolamine-binding protein 1 preproprotein
• NCBI Official Synonym Full Names: phosphatidylethanolamine binding protein 1
• NCBI Official Symbol: PEBP1
• NCBI Official Synonym Symbols: PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1
• NCBI Protein Information: phosphatidylethanolamine-binding protein 1; neuropolypeptide h3; prostatic binding protein; prostatic-binding protein; raf kinase inhibitor protein; Raf kinase inhibitory protein; hippocampal cholinergic neurostimulating peptide
• UniProt Protein Name: Phosphatidylethanolamine-binding protein 1
• Protein Family: Phosphatidylethanolamine-binding protein
• UniProt Gene Name: PEBP1
• UniProt Synonym Gene Names: PBP; PEBP; PEBP-1; RKIP; HCNP
• UniProt Entry Name: PEBP1_HUMAN

NCBI Description

This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Jul 2015]

Uniprot Description

RKIP: a metastasis suppressor that regulates extracellular matrix remodeling and tumor survival. Binds to and inhibits Raf-1. Phosphorylation by classic and atypical but not novel PKC isoforms dissociates this complex, relieving inhibition of the Raf/MAP kinase signaling cascade. Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. Cleaved into hippocampal cholinergic neurostimulating peptide (HCMP) that appears to be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Expressed in brain. Increased expression in aged senescence-accelerated mice.

Protein type: Lipid-binding; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 12q24.23

Cellular Component: cytoplasm; nucleus

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; phosphatidylethanolamine binding; protein kinase binding; ATP binding