PEBP1 recombinant protein

PEBP1, 1-187aa, Human, E Coli

Cat. Num. AAA203383

• Gene Names: PEBP1; PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1; HEL-210; HEL-S-34; HEL-S-96
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: PEBP1; 1-187aa; Human; E Coli; Phosphatidylethanolamine binding protein 1; HCNP; PBP; PEBP; Phosphatidylethanolamine binding protein 1 Hippocampal cholinergic neurostimulating peptide; Neuropolypeptide h3; HCNPpp; PEBP 1; Phosphatidylethanolamine binding protein; Prostatic binding protein; R kip; Raf kinase inhibitory protein RKIP.; PEBP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MPVDLSKWSG PLSLQEVDEQ PQHPLHVTYA GAAVDELGKV LTPTQVKNRP TSISWDGLDS GKLYTLVLTD PDAPSRKDPK YREWHHFLVV NMKGNDISSG TVLSDYVGSG PPKGTGLHRY VWLVYEQDRP LKCDEPILSN RSGDHRGKFK VASFRKKYEL RAPVAGTCYQ AEWDDYVPKL YEQLSGK
• Sequence Length: 187

• Applicable Applications for PEBP1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PEBP1 recombinant protein

Phosphatidylethanolamine binding protein 1 (PEBP1), also known as Raf kinase inhibitor protein (RKIP), is a member of the phosphatidylethanolamine-binding protein family and a serine protease inhibitor which inhibits thrombin, neuropsin. PEBP1 plays a pivotal modulatory role in several protein kinase signaling cascades. Protein kinase C (PKC) phosphorylates PEBP1, resulting in release of Raf-1 and activation of MEK and ERK. Expressed in many tissues, PEBP1 is implicated in the regulation of such physiological processes as membrane biosynthesis, spermatogenesis, neural development, and metastasis suppression. Recombinant human PEBP1 was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PEBP1 recombinant protein

Proteases & Inhibitors

References

Keller ET., et al. (2004) Biochem Pharmacol. 68 (6):1049-53. ; Park S., et al. (2005) Oncogene. 24 (21):3535-40. ;

NCBI and Uniprot Product Information

• NCBI GI #: 4505621
• NCBI GeneID: 5037
• NCBI Accession #: NP_002558
• NCBI GenBank Nucleotide #: NM_002567.3
• UniProt Accession #: P30086; B2R4S1
• Molecular Weight: 21 kDa (187aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: phosphatidylethanolamine-binding protein 1
• NCBI Official Synonym Full Names: phosphatidylethanolamine binding protein 1
• NCBI Official Symbol: PEBP1
• NCBI Official Synonym Symbols: PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1; HEL-210; HEL-S-34; HEL-S-96
• NCBI Protein Information: phosphatidylethanolamine-binding protein 1
• UniProt Protein Name: Phosphatidylethanolamine-binding protein 1
• Protein Family: Phosphatidylethanolamine-binding protein
• UniProt Gene Name: PEBP1
• UniProt Synonym Gene Names: PBP; PEBP; PEBP-1; RKIP; HCNP

NCBI Description

This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Jul 2015]

Uniprot Description

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation.