Western Blot (WB)
(Figure 1. Western blot analysis of Profilin 2/PFN2 using anti-Profilin 2/PFN2 antibody (MBS1753737).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human U87 whole cell lysatesLane 2: human PC-3 whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: human Hela whole cell lysatesLane 5: human HEK293 whole cell lysatesLane 6: rat brain tissue lysatesLane 7: mouse brain tissue lysatesLane 8: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Profilin 2/PFN2 antigen affinity purified polyclonal antibody (Catalog # MBS1753737) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Profilin 2/PFN2 at approximately 15KD. The expected band size for Profilin 2/PFN2 is at 15KD. )
Rabbit Profilin 2/PFN2 Polyclonal Antibody | anti-PFN2 antibody
Anti-Profilin 2/PFN2 Antibody
IHC-P: 2-5ug/ml|Human, Mouse, Rat|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of Profilin 2/PFN2 using anti-Profilin 2/PFN2 antibody (MBS1753737).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human U87 whole cell lysatesLane 2: human PC-3 whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: human Hela whole cell lysatesLane 5: human HEK293 whole cell lysatesLane 6: rat brain tissue lysatesLane 7: mouse brain tissue lysatesLane 8: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Profilin 2/PFN2 antigen affinity purified polyclonal antibody (Catalog # MBS1753737) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Profilin 2/PFN2 at approximately 15KD. The expected band size for Profilin 2/PFN2 is at 15KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Profilin 2/PFN2 using anti-Profilin 2/PFN2 antibody (MBS1753737).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human U87 whole cell lysatesLane 2: human PC-3 whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: human Hela whole cell lysatesLane 5: human HEK293 whole cell lysatesLane 6: rat brain tissue lysatesLane 7: mouse brain tissue lysatesLane 8: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Profilin 2/PFN2 antigen affinity purified polyclonal antibody (Catalog # MBS1753737) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Profilin 2/PFN2 at approximately 15KD. The expected band size for Profilin 2/PFN2 is at 15KD. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Profilin 2/PFN2 using anti-Profilin 2/PFN2 antibody (MBS1753737).Profilin 2/PFN2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Profilin 2/PFN2 Antibody (MBS1753737) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Profilin 2/PFN2 using anti-Profilin 2/PFN2 antibody (MBS1753737).Profilin 2/PFN2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Profilin 2/PFN2 Antibody (MBS1753737) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of Profilin 2/PFN2 using anti-Profilin 2/PFN2 antibody (MBS1753737).Profilin 2/PFN2 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Profilin 2/PFN2 Antibody (MBS1753737) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of Profilin 2/PFN2 using anti-Profilin 2/PFN2 antibody (MBS1753737).Profilin 2/PFN2 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Profilin 2/PFN2 Antibody (MBS1753737) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of Profilin 2/PFN2 using anti-Profilin 2/PFN2 antibody (MBS1753737).Profilin 2/PFN2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Profilin 2/PFN2 Antibody (MBS1753737) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of Profilin 2/PFN2 using anti-Profilin 2/PFN2 antibody (MBS1753737).Profilin 2/PFN2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Profilin 2/PFN2 Antibody (MBS1753737) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Flow Cytometry (FC/FACS)
(Figure 5. Flow Cytometry analysis of A431 cells using anti-Profilin 2/PFN2 antibody (MBS1753737).Overlay histogram showing A431 cells stained with MBS1753737 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Profilin 2/PFN2 Antibody (MBS1753737, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 5. Flow Cytometry analysis of A431 cells using anti-Profilin 2/PFN2 antibody (MBS1753737).Overlay histogram showing A431 cells stained with MBS1753737 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Profilin 2/PFN2 Antibody (MBS1753737, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Joensuu, T., Blanco, G., Pakarinen, L., Sistonen, P., Kaariainen, H., Brown, S., de la Chapelle, A., Sankila, E. -M. Refined mapping of the Usher syndrome type III locus on chromosome 3, exclusion of candidate genes, and identification of the putative mouse homologous region. Genomics 38: 255-263, 1996.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
profilin 2: Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Belongs to the profilin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Actin-binding
Chromosomal Location of Human Ortholog: 3q25.1
Cellular Component: cytoskeleton; cytoplasm; terminal button
Molecular Function: actin monomer binding; protein binding; phosphatidylinositol-4,5-bisphosphate binding; adenyl-nucleotide exchange factor activity
Biological Process: positive regulation of actin filament polymerization; positive regulation of ATPase activity; negative regulation of actin filament polymerization; positive regulation of stress fiber formation; positive regulation of actin filament bundle formation; actin cytoskeleton organization and biogenesis; positive regulation of peptidyl-serine phosphorylation
Research Articles on PFN2
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Product Notes
The PFN2 pfn2 (Catalog #AAA1753737) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Profilin 2/PFN2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Profilin 2/PFN2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.1-0.25ug/ml|Human, Mouse, Rat| IHC-P: 2-5ug/ml|Human, Mouse, Rat| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the PFN2 pfn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Profilin 2/PFN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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