Western Blot (WB)
(Figure 1. Western blot analysis of TRPV4 using anti-TRPV4 antibody (MBS1753345).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: rat PC-12 whole cell lysatesLane 3: rat C6 whole cell lysatesLane 4: mouse brain tissue lysatesLane 5: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-TRPV4 antigen affinity purified polyclonal antibody (Catalog # MBS1753345) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for TRPV4 at approximately 98KD. The expected band size for TRPV4 is at 98KD. )
Rabbit TRPV4 Polyclonal Antibody | anti-TRPV4 antibody
Anti-TRPV4 Antibody
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of TRPV4 using anti-TRPV4 antibody (MBS1753345).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: rat PC-12 whole cell lysatesLane 3: rat C6 whole cell lysatesLane 4: mouse brain tissue lysatesLane 5: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-TRPV4 antigen affinity purified polyclonal antibody (Catalog # MBS1753345) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for TRPV4 at approximately 98KD. The expected band size for TRPV4 is at 98KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of TRPV4 using anti-TRPV4 antibody (MBS1753345).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: rat PC-12 whole cell lysatesLane 3: rat C6 whole cell lysatesLane 4: mouse brain tissue lysatesLane 5: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-TRPV4 antigen affinity purified polyclonal antibody (Catalog # MBS1753345) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for TRPV4 at approximately 98KD. The expected band size for TRPV4 is at 98KD. )
2. Strotmann, R., Harteneck, C., Nunnenmacher, K., Schultz, G., Plant, T. D. OTRPC4, a nonselective cation channel that confers sensitivity to extracellular osmolarity. Nature Cell. Biol. 2: 695-702, 2000.
3. Wissenbach, U., Bodding, M., Freichel, M., Flockerzi, V. Trp12, a novel Trp related protein from kidney FEBS Lett. 485: 127-134, 2000.
NCBI and Uniprot Product Information
Observed: 110 kDa
NCBI Description
This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in this gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]
Uniprot Description
TRPV4: a non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Channel, cation; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12q24.1
Cellular Component: cortical actin cytoskeleton; cell surface; focal adhesion; growth cone; adherens junction; lamellipodium; cytoplasmic microtubule; plasma membrane; integral to membrane; cytoplasmic vesicle; filopodium; cilium
Molecular Function: calcium channel activity; microtubule binding; beta-tubulin binding; actin binding; protein kinase binding; calmodulin binding; actin filament binding; protein binding; protein kinase C binding; osmosensor activity; SH2 domain binding; cation channel activity; alpha-tubulin binding; ATP binding
Biological Process: intercellular junction assembly; actin filament organization; regulation of response to osmotic stress; osmosensory signaling pathway; cellular calcium ion homeostasis; vasopressin secretion; positive regulation of microtubule depolymerization; elevation of cytosolic calcium ion concentration; hyperosmotic salinity response; response to mechanical stimulus; calcium ion transport; actin cytoskeleton reorganization; cell volume homeostasis; cortical microtubule organization and biogenesis; transmembrane transport; microtubule polymerization
Disease: Brachyolmia Type 3; Scapuloperoneal Spinal Muscular Atrophy; Hereditary Motor And Sensory Neuropathy, Type Iic; Sodium Serum Level Quantitative Trait Locus 1; Spondyloepiphyseal Dysplasia, Maroteaux Type; Parastremmatic Dwarfism; Spinal Muscular Atrophy, Distal, Congenital Nonprogressive; Digital Arthropathy-brachydactyly, Familial; Metatropic Dysplasia; Spondylometaphyseal Dysplasia, Kozlowski Type
Research Articles on TRPV4
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Product Notes
The TRPV4 trpv4 (Catalog #AAA1753345) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TRPV4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRPV4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse, Rat| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the TRPV4 trpv4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRPV4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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